Journal of Medical Entomology (2016) 53, 1348-1363

From Pestinfo-Wiki
Jump to: navigation, search

Yvonne Ukamaka Ajamma, Jandouwe Villinger, David Omondi, Daisy Salifu, Thomas Ogao Onchuru, Laban Njoroge, Anne W.T. Muigai and Daniel K. Masiga (2016)
Composition and genetic diversity of mosquitoes (Diptera: Culicidae) on islands and mainland shores of Kenya's Lakes Victoria and Baringo
Journal of Medical Entomology 53 (6), 1348-1363
Abstract: The Lake Baringo and Lake Victoria regions of Kenya are associated with high seroprevalence of mosquito-transmitted arboviruses. However, molecular identification of potential mosquito vector species, including morphologically identified ones, remains scarce. To estimate the diversity, abundance, and distribution of mosquito vectors on the mainland shores and adjacent inhabited islands in these regions, we collected and morphologically identified adult and immature mosquitoes and obtained the corresponding sequence variation at cytochrome c oxidase 1 (COI) and internal transcribed spacer region 2 (ITS2) gene regions. A total of 63 species (including five subspecies) were collected from both study areas, 47 of which have previously been implicated as disease vectors. Fourteen species were found only on island sites, which are rarely included in mosquito diversity surveys. We collected more mosquitoes, yet with lower species composition, at Lake Baringo (40,229 mosquitoes, 32 species) than at Lake Victoria (22,393 mosquitoes, 54 species). Phylogenetic analysis of COI gene sequences revealed Culex perexiguus and Cx. tenagius that could not be distinguished morphologically. Most Culex species clustered into a heterogeneous clade with closely related sequences, while Culex pipiens clustered into two distinct COI and ITS2 clades. These data suggest limitations in current morphological identification keys. This is the first DNA barcode report of Kenyan mosquitoes. To improve mosquito species identification, morphological identifications should be supported by their molecular data, while diversity surveys should target both adults and immatures. The diversity of native mosquito disease vectors identified in this study impacts disease transmission risks to humans and livestock.
(The abstract is excluded from the Creative Commons licence and has been copied with permission by the publisher.)
Full text of article


Research topic(s) for pests/diseases/weeds:
surveys/sampling/distribution


Pest and/or beneficial records:

Beneficial Pest/Disease/Weed Crop/Product Country Quarant.


Anopheles gambiae Kenya
Culex pipiens Kenya
Aedes aegypti Kenya
Anopheles funestus Kenya
Anopheles pharoensis Kenya
Anopheles coustani Kenya
Anopheles ziemanni Kenya
Mimomyia splendens Kenya
Aedes vittatus Kenya
Culex univittatus Kenya
Anopheles rhodesiensis Kenya
Anopheles squamosus Kenya
Culex antennatus Kenya
Culex poicilipes Kenya
Culex tigripes Kenya
Anopheles tenebrosus Kenya
Culex neavei Kenya
Aedes luteocephalus Kenya
Aedes cumminsii Kenya
Aedes tarsalis Kenya
Aedes metallicus Kenya
Culex zombaensis Kenya
Culex simpsoni Kenya
Culex thalassius Kenya
Culex nebulosus Kenya
Anopheles symesi Kenya
Coquillettidia metallica Kenya