Pesquisa Agropecuária Brasileira (1999) 34, 1497-1503
Marcos Rodrigues De Faria, Irene Martins, Raquel Mello and Myrian Silvana Tigano (1999)
Entomopathogenic fungal (Hyphomycetes) collection: assessment of conidial viability
Pesquisa Agropecuária Brasileira 34 (8), 1497-1503
Abstract: Twenty four strains of the entomopathogenic fungi (Hyphomycetes) Beauveria bassiana, Metarrhizium anisopliae, Nomuraea rileyi, Paecilomyces farinosus, P. fumosoroseus and P. lilacinus, maintained in the culture collection of Embrapa-Centro Nacional de Pesquisa de Recursos Genéticos e Biotecnologia (Cenargen) and preserved by lyophilization and in liquid nitrogen, had their conidial viability assessed. Germination rates of 16- to 84-month-old cultures stored in liquid nitrogen decreased, on average, less than 13.3%. For 29- to 49-month-old cultures preserved by lyophilization, the viability loss ranged, on average, from 28.6 to 94.5%. The results demonstrated the efficiency of the tested methods, especially liquid nitrogen, in preserving the viability of entomopathogenic fungi.
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Database assignments for author(s): Myrian S. Tigano
Research topic(s) for pests/diseases/weeds:
biocontrol - natural enemies
Research topic(s) for beneficials or antagonists:
general biology - morphology - evolution
rearing/culturing/mass production
