Applied and Environmental Microbiology (2000) 66, 4378-4382

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Emma T. Steenkamp, Brenda D. Wingfield, Teresa A. Coutinho, Kurt A. Zeller, Michael J. Wingfield, Walter F.O. Marasas and John F. Leslie (2000)
PCR-based identification of MAT-1 and MAT-2 in the Gibberella fujikuroi species complex
Applied and Environmental Microbiology 66 (10), 4378-4382
Abstract: All sexually fertile strains in the Gibberella fujikuroi species complex are heterothallic, with individual mating types conferred by the broadly conserved ascomycete idiomorphs MAT-1 and MAT-2. We sequenced both alleles from all eight mating populations, developed a multiplex PCR technique to distinguish these idiomorphs, and tested it with representative strains from all eight biological species and 22 additional species or phylogenetic lineages from this species complex. In most cases, either an ~800-bp fragment from MAT-2 or an ~200-bp fragment from MAT-1 is amplified. The amplified fragments cosegregate with mating type, as defined by sexual cross-fertility, in a cross of Fusarium moniliforme (Fusarium verticillioides). Neither of the primer pairs amplify fragments from Fusarium species such as Fusarium graminearum, Fusarium pseudograminearum, and Fusarium culmorum, which have, or are expected to have, Gibberella sexual stages but are thought to be relatively distant from the species in the G. fujikuroi species complex. Our results suggest that MAT allele sequences are useful indicators of phylogenetic relatedness in these and other Fusarium species.
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Database assignments for author(s): Brenda D. Wingfield, Michael J. Wingfield, Teresa A. Coutinho, John F. Leslie

Research topic(s) for pests/diseases/weeds:

Pest and/or beneficial records:

Beneficial Pest/Disease/Weed Crop/Product Country Quarant.

Panama disease of banana
Neocosmospora solani
Fusarium oxysporum f.sp. niveum
Fusarium culmorum
Fusarium avenaceum
Fusarium graminearum
Fusarium verticillioides
Fusarium crown and root rot
Fusarium gossypinum
Fusarium acuminatum
Fusarium subglutinans Mango (Mangifera indica)
Fusarium oxysporum f.sp. chrysanthemi
Fusarium pseudograminearum
Fusarium cerealis
Fusarium oxysporum