Systematic Entomology (2003) 28, 57-69
Bin Chen, Roger K. Butlin and Ralph E. Harbach (2003)
the Myzomyia Series of Anopheles subgenus Cellia (Diptera: Culicidae) inferred from nuclear and mitochondrial DNA sequences
Systematic Entomology 28 (1), 57-69
Abstract: The phylogenetic relationships of fifteen Oriental and two Afrotropical taxa of the Myzomyia Series of Anopheles subgenus Cellia and two outgroup species, An. maculatus (Neocellia Series) and An. dirus A (Neomyzomyia Series), were inferred from nucleotide sequences of the entire 685 bp of the mitochondrial cytochrome oxidase subunit II locus (COII) and 372 bp of the third domain (D3) of the 28S rDNA locus, both separately and together. Alignment of the D3 sequences was achieved with the aid of secondary structure comparisons, and the pattern of nucleotide substitution was best explained by the GTR + I + G model for either separate or combined datasets. Maximum likelihood and maximum parsimony analyses robustly identified five monophylies: An. fluviatilis U and T; An. fluviatilis U and T + An. minimus A, C, E and #157 + An. leesoni; An. filipinae + An. mangyanus; An. filipinae + An. mangyanus + An. aconitus; and An. culicifacies A and B. The results confirm the specific status of An. flavirostris, the close relationship of An. leesoni with the Minimus Complex, and the exclusion of An. jeyporiensis, An. culicifacies s.l and An. funestus from the Minimus Group. All of the species classified as members of the Minimus Group on morphological grounds formed a single clade, which comprised two subgroups: the Minimus Subgroup, including An. minimus s.l., An. fluviatilis s.l., An. leesoni and An. flavirostris, and the Aconitus Subgroup, including An. filipinae, An. mangyanus, An. aconitus, An. pampanai and An. varuna. However, these clades are only weakly supported by the present dataset.
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Link to article at publishers website
Database assignments for author(s): Bin Chen, Ralph E. Harbach
Research topic(s) for pests/diseases/weeds:
identification/taxonomy
Pest and/or beneficial records:
| Beneficial | Pest/Disease/Weed | Crop/Product | Country | Quarant.
|
|---|---|---|---|---|
| Anopheles (genus) | ||||
| Anopheles varuna | ||||
| Anopheles culicifacies | ||||
| Anopheles maculatus | ||||
| Anopheles dirus | ||||
| Anopheles minimus | ||||
| Anopheles fluviatilis | ||||
| Anopheles flavirostris | ||||
| Anopheles aconitus | ||||
| Anopheles pampanai |
