PLoS ONE (2020) 15 (8 - e0234098)

Sébastien Briolant, Monique Melo Costa, Christophe Nguyen, Isabelle Dusfour, Vincent Pommier de Santi, Romain Girod and Lionel Almeras (2020)
Identification of French Guiana anopheline mosquitoes by MALDI-TOF MS profiling using protein signatures from two body parts
PLoS ONE 15 (8 - e0234098)
Abstract: In French Guiana, the malaria, a parasitic infection transmitted by Anopheline mosquitoes, remains a disease of public health importance. To prevent malaria transmission, the main effective way remains Anopheles control. For an effective control, accurate Anopheles species identification is indispensable to distinguish malaria vectors from non-vectors. Although, morphological and molecular methods are largely used, an innovative tool, based on protein pattern comparisons, the Matrix Assisted Laser Desorption / Ionization Time-of-Flight Mass Spectrometry (MALDI-TOF MS) profiling, emerged this last decade for arthropod identification. However, the limited mosquito fauna diversity of reference MS spectra remains one of the main drawback for its large usage. The aim of the present study was then to create and to share reference MS spectra for the identification of French Guiana Anopheline species. A total of eight distinct Anopheles species, among which four are malaria vectors, were collected in 6 areas. To improve Anopheles identification, two body parts, legs and thoraxes, were independently submitted to MS for the creation of respective reference MS spectra database (DB). This study underlined that double checking by MS enhanced the Anopheles identification confidence and rate of reliable classification. The sharing of this reference MS spectra DB should make easier Anopheles species monitoring in endemic malaria area to help malaria vector control or elimination programs.
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Database assignments for author(s): Isabelle Dusfour, Romain Girod

Research topic(s) for pests/diseases/weeds:
surveys/sampling/distribution
identification/taxonomy

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Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
	Anopheles aquasalis		French Guiana
	Anopheles darlingi		French Guiana
	Anopheles triannulatus		French Guiana
	Anopheles nuneztovari		French Guiana
	Anopheles braziliensis		French Guiana
	Anopheles oswaldoi		French Guiana
	Anopheles intermedius		French Guiana