Applied Entomology and Zoology (2013) 48, 469-476

Chayanit Hempolchom, Yasushi Otsuka, Visut Baimai, Sorawat Thongsahuan, Atiporn Saeung, Kritsana Taai, Wichai Srisuka, Pradya Somboon and Wej Choochote (2013)
Development of a multiplex PCR assay for the identification of eight species members of the Thai Hyrcanus Group (Diptera: Culicidae)
Applied Entomology and Zoology 48 (4), 469-476
Abstract: The Hyrcanus Group comprises important vectors of malaria because of Plasmodium vivax Grassi and Feletti and filariasis caused by Brugia malayi Brug in many countries of South, Southeast and East Asian regions. In Thailand, eight species members (Anopheles argyropus Swellengrebel, An. crawfordi Reid, An. nigerrimus Giles, An. nitidus Harrison, Scanlon and Reid, An. paraliae Sandosham, An. peditaeniatus Leicester, An. pursati Laveran and An. sinensis Wiedemann) of the Hyrcanus Group have been recognized. Due to morphological overlap, adult females of the Hyrcanus Group in Thailand have been misidentified markedly among the eight species, particularly when using the traumatic scales of wild-caught specimens from epidemiology and control approaches. Therefore, this study first developed a simple and robust multiplex PCR assay, based on second internal transcribed spacer sequences of ribosomal DNA, for differentiating the eight species members of the Thai Hyrcanus Group.
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Link to article at publishers website
Database assignments for author(s): Pradya Somboon, Atiporn Saeung

Research topic(s) for pests/diseases/weeds:
identification/taxonomy
surveys/sampling/distribution

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Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
	Anopheles peditaeniatus		Thailand
	Anopheles nigerrimus		Thailand
	Anopheles sinensis		Thailand
	Anopheles paraliae		Thailand
	Anopheles argyropus		Thailand
	Anopheles crawfordi		Thailand
	Anopheles nitidus		Thailand
	Anopheles pursati		Thailand