Applied Entomology and Zoology (2011) 46, 301-309
Oumi Nishi, Keiichi Hasegawa, Kazuhiro Iiyama, Chisa Yasunaga-Aoki and Susumu Shimizu (2011)
Phylogenetic analysis of Metarhizium spp. isolated from soil in Japan
Applied Entomology and Zoology 46 (3), 301-309
Abstract: As a result of analyzing the internal transcribed spacer (ITS) and 5' end of the EF-1α sequence of 145 isolates of Metarhizium spp. isolated from soil in Japan using selective agar medium, eight species were identified. ITS sequence analysis divided the isolates into three clades. Two were identified as M. flavoviride var. pemphigi and M. lepidiotae, respectively. EF-1α sequence analysis identified the other clades as six species: M. anisopliae, M. brunneum, M. guizhouense, M. majus, M. pingshaense and M. robertisii. The distribution of M. flavoviride var. pemphigi was restricted to forest or wood soil, and conidial sizes of M. guizhouense and M. majus were incongruent with the phylogeny based on the sequence of the 5' end of EF-1α.
(The abstract is excluded from the Creative Commons licence and has been copied with permission by the publisher.)
(original language: English)
Link to article at publishers website
Database assignments for author(s): Oumi Nishi
Research topic(s) for pests/diseases/weeds:
biocontrol - natural enemies
Research topic(s) for beneficials or antagonists:
identification/taxonomy
molecular biology - genes
