Neotropical Entomology (2018) 47, 292-301

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L.M. Silva, M.C. Silva, S.M.F.A. Silva, R.C. Alves, H.A.A. Siqueira and E.J. Marques (2018)
Toxin gene contents and activity of Bacillus thuringiensis strains against two sugarcane borer species, Diatraea saccharalis (F.) and D. flavipennella (Box)
Neotropical Entomology 47 (2), 292-301
Abstract: Bacillus thuringiensis (Berliner) bears essential characteristics in the control of insect pests, such as its unique mode of action, which confers specificity and selectivity. This study assessed cry gene contents from Bt strains and their entomotoxicity against Diatraea saccharalis (F.) and Diatraea flavipennella (Box) (Lepidoptera: Crambidae). Bioassays with Bt strains were performed against neonates to evaluate their lethal and sublethal activities and were further analyzed by PCR, using primers to identify toxin genes. For D. saccharalis and D. flavipennella, 16 and 18 strains showed over 30% larval mortality in the 7th day, respectively. The LC50 values of strains for D. saccharalis varied from 0.08 × 105 (LIIT-0105) to 4104 × 105 (LIIT-2707) spores + crystals mL-1. For D. flavipennella, the LC50 values of strains varied from 0.40 × 105 (LIIT-2707) to 542 × 105 (LIIT-2109) spores + crystals mL-1. For the LIIT-0105 strain, which was the most toxic to D. saccharalis, the genes cry1Aa, cry1Ab, cry1Ac, cry1B, cry1C, cry1D, cry1F, cry1I, cry2Aa, cry2Ab, cry8, and cry9C were detected, whereas for the strain LIIT-2707, which was the most toxic to D. flavipennella, detected genes were cry1Aa, cry1Ab, cry1Ac, cry1B, cry1D, cry1F, cry1I, cry2Aa, cry2Ab, and cry9. The toxicity data and toxin gene content in these strains of Bt suggest a great variability of activity with potential to be used in the development of novel biopesticides or as source of resistance genes that can be expressed in plants to control pests.
(The abstract is excluded from the Creative Commons licence and has been copied with permission by the publisher.)
Link to article at publishers website
Database assignments for author(s): Herbert A.A. Siqueira, Edmilson J. Marques

Research topic(s) for pests/diseases/weeds:
biocontrol - natural enemies
Research topic(s) for beneficials or antagonists:
evaluation - screening - selection

Pest and/or beneficial records:

Beneficial Pest/Disease/Weed Crop/Product Country Quarant.

Diatraea saccharalis
Diatraea flavipennella
Bacillus thuringiensis Cry1A-toxin (entomopathogen) Diatraea saccharalis
Bacillus thuringiensis Cry1A-toxin (entomopathogen) Diatraea flavipennella
Bacillus thuringiensis Cry1D-toxin (entomopathogen) Diatraea saccharalis
Bacillus thuringiensis Cry1D-toxin (entomopathogen) Diatraea flavipennella
Bacillus thuringiensis Cry1F-toxin (entomopathogen) Diatraea saccharalis
Bacillus thuringiensis Cry1F-toxin (entomopathogen) Diatraea flavipennella
Bacillus thuringiensis Cry1B-toxin (entomopathogen) Diatraea saccharalis
Bacillus thuringiensis Cry1B-toxin (entomopathogen) Diatraea flavipennella
Bacillus thuringiensis Cry1C-toxin (entomopathogen) Diatraea saccharalis
Bacillus thuringiensis Cry2A-toxin (entomopathogen) Diatraea saccharalis
Bacillus thuringiensis Cry2A-toxin (entomopathogen) Diatraea flavipennella
Bacillus thuringiensis Cry9C-toxin (entomopathogen) Diatraea saccharalis
Bacillus thuringiensis Cry1I-toxin (entomopathogen) Diatraea flavipennella