Journal für Kulturpflanzen - Journal of Cultivated Plants (2018) 70, 95-107

Eckhard Koch, Petra Zink, Cornelia I. Ullrich and Regina G. Kleespies (2018)
Light microscopic studies on the development of Beauveria bassiana and other putative endophytes in leaf tissues
Journal für Kulturpflanzen - Journal of Cultivated Plants 70 (3), 95-107
Abstract: The study involved six test fungi previously recorded in the literature as being endophytes ( Beauveria bassiana , Metarhizium anisopliae , Isaria fumosorosea, Trichoderma harzianum, Fusarium proliferatum , Chaetomium globosum ), two plant pathogenic fungi ( Ascochyta fabae , Plenodomus lingam ) and four host plants ( Vicia faba, Brassica napus, Phaseolus vulgaris, Zea mays) . Aerial conidia, blastospores, or ascospores, respectively were applied to leaf surfaces by spraying or by infiltrating spore suspensions through stomata directly into the leaves. Observations using light microscopy showed that the test fungi germinated on the leaf surface but did not enter actively into the leaves. Within the leaves, germination of spores and growth of hyphae appeared to depend on the presence of damaged plant tissue. Various host reactions such as browning of epidermal cells and formation of papillae were observed. Colonization of healthy leaves by the test fungi in a manner similar to the pathogens A. fabae (on Faba bean) and P. lingam (on oilseed rape) was not observed. Spore germination and hyphal growth commenced when inoculated leaves were placed on agar medium. The results indicate that the test fungi possessed a saprotrophic rather than an endophytic life style when associated with leaf tissue of the studied hosts.
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Link to article at publishers website
Database assignments for author(s): Eckhard Koch, Regina G. Kleespies

Research topic(s) for pests/diseases/weeds:
biocontrol - natural enemies
Research topic(s) for beneficials or antagonists:
general biology - morphology - evolution

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Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
	Ascochyta fabae	Broad/faba bean (Vicia faba)
	Plenodomus lingam	Rape/canola (Brassica napus)
Beauveria bassiana (entomopathogen)
Cordyceps fumosorosea (entomopathogen)
Metarhizium anisopliae (entomopathogen)
Trichoderma harzianum (antagonist)
Chaetomium globosum (antagonist)