Formosan Entomologist (2004) 24, 159-171
Yi-Chung Chiu, Wen-Je Wur, Cheng-Yu Wong, Shu-Pei Chen and Cheng-Jen Shih (2004)
[Application of the PCR-RFLP technique for the rapid diagnosis of scale insects (Homoptera: Coccoidea) on imported agricultural products in Taiwan]
Formosan Entomologist 24 (2), 159-171
Abstract: There are 30 scale insect species which have been intercepted from agricultural products coming into Taiwan by quarantine workers at customs. This study attempted to develop a molecular diagnostic technique (polymerase chain reaction-restriction fragment length polymorphism, PCR- RFLP) using genomic DNA markers (ribosomal DNA, rDNA) for identifying 16 scale insect species. NS7 and ITS6 primer sets were used to amplify ITS1 and its flanking regions of rDNA from genomic DNA as a template extracted from a single specimen. DNA extracted from these 16 scale insect species yielded a single fragment after PCR amplification. These PCR products were then cut with various restriction endonucleases in order to compare the results of restriction fragment length polymorphism. Our results also reveal that it is possible to discriminate these 16 scale insect species based on the species-specific patterns acquired from digesting the PCR products with the endonucleases, TaqI, MspI, and HaeIII. Futhermore, we also a set up a molecular key for these 16 scale insect species based on their RFLP digestion patterns.
(The abstract is excluded from the Creative Commons licence and has been copied with permission by the publisher.)
(original language: Chinese)
Research topic(s) for pests/diseases/weeds:
identification/taxonomy
quarantine treatments/regulations/aspects
