Forest Pathology (2020) 50 (6 - e12645)

Minette Havenga, Brenda D. Wingfield, Michael J. Wingfield, Léanne L. Dreyer, Francois Roets and Janneke Aylward (2020)
Diagnostic markers for Teratosphaeria destructans and closely related species
Forest Pathology 50 (6 - e12645)
Abstract: Teratosphaeria foliar pathogens cause leaf and shoot blight on Eucalyptus trees in many parts of the world. Among them, T. destructans is one of the most aggressive pathogens causing defoliation of young Eucalyptus trees in tropic regions. Identification of T. destructans to species level is currently not possible based solely on morphological characteristics or ITS sequence data. The aim of this study was to assess T. destructans microsatellites and a newly developed T. epicoccoides microsatellite as a diagnostic tool to differentiate among T. destructans and several closely related foliar pathogens. Based on the number of markers that amplified, the T. destructans microsatellites allowed for the differentiation of T. destructans, T. epicoccoides,T. eucalypti,T. nubilosa,T. pseudoeucalypti and T. viscidus. These microsatellites provide a rapid and cost-effective diagnostic tool that will enable the identification of a large number of isolates important in disease surveys and inoculation trials.
(The abstract is excluded from the Creative Commons licence and has been copied with permission by the publisher.)
Link to article at publishers website
Database assignments for author(s): Brenda D. Wingfield, Michael J. Wingfield, Francois Roets, Janneke Aylward

Research topic(s) for pests/diseases/weeds:
identification/taxonomy
molecular biology - genes

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Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
	Teratosphaeria nubilosa
	Teratosphaeria eucalypti
	Teratosphaeria epicoccoides
	Teratosphaeria destructans	Eucalypt (Eucalyptus)
	Teratosphaeria viscida
	Teratosphaeria pseudoeucalypti