Forest Pathology (2012) 42, 22-27
A. Rytkönen, A. Lilja and J. Hantula (2012)
PCR-DGGE method for in planta detection and identification of Phytophthora species
Forest Pathology 42 (1), 22-27
Abstract: We have developed a method to detect multiple species of Phytophthora directly in infected plant tissues. The method is based on the polymerase chain reaction and uses Phytopththora-specific primers and denaturing gradient gel electrophoresis (PCR-DGGE). The method distinguished most of the 16 Phytophthora species tested. Very closely related species might not, however, be identified using the method. The detection efficiency was high and successful in eight different plant tissues tested. The PCR-DGGE detection tool developed here will be a fast and inexpensive method suitable for pathogen surveys and research programmes.
(The abstract is excluded from the Creative Commons licence and has been copied with permission by the publisher.)
Link to article at publishers website
Database assignments for author(s): Anna Rytkönen, Jarkko Hantula, Arja Lilja
Research topic(s) for pests/diseases/weeds:
identification/taxonomy
surveys/sampling/distribution
