View source for Plant Disease (2018) 102, 154-164

{{Publication
|Publication authors=Sabina Zoledowska, Agata Motyka, Dominika Zukowska, Wojciech Sledz and [[Ewa Lojkowska]]
|Author Page=Ewa Lojkowska
|Publication date=2018
|dc:title= Population structure and biodiversity of ''[[Pectobacterium parmentieri]]'' isolated from potato fields in temperate climate
|Publication journal=Plant Disease
|prism:volume=102 (1)
|prism:startingPage=154
|Last page=164
|DOI=10.1094/PDIS-05-17-0761-RE
|Publication abstract=''Pectobacterium parmentieri'' (formerly ''Pectobacterium wasabiae'') is a newly established species of pectinolytic plant-pathogenic bacteria responsible for the symptoms of soft rot and blackleg on potato. In this work, we describe biodiversity and the population structure of ''P. parmentieri'' strains isolated during two consecutive growing seasons from the seed potato fields in Poland. About 450 samples of diseased potato tubers, potato plants, or accompanying weeds were collected throughout the country and tested for the presence of ''P. parmentieri'' by molecular identification methods. We found that ''P. parmentieri'' strains commonly occur in almost all regions of Poland. Furthermore, these isolates constituted significant fraction of pectinolytic bacteria from seed potato fields because 16% (2013) and 13% (2014) of the analyzed plant samples were infected with ''P. parmentieri''. Subsequently, a detailed characterization of the obtained strains was conducted basing on repetitive sequences profiling, ''recA''-gene-based phylogeny, and phenotypic features. By applying repetitive extragenic palindromic sequence-based polymerase chain reaction (REP-PCR), we revealed the presence of five distinct genomic profiles among ''P. parmentieri'' strains, with profile I being the most abundant (approximately 44%). The performed ''recA'' gene-based phylogenetic analysis divided ''P. parmentieri'' isolates into two distinct clades, although the strains originating from different years did not group separately. Evaluation of the phenotypic traits playing crucial roles for the virulence of pectinolytic bacteria (namely, pectinase, cellulase and protease activities, and siderophore production, in addition to potato tissue maceration, swimming, and swarming motility) indicated some differences among the characterized strains. To the best of our knowledge, this is the first study that describes biodiversity and the population structure of ''P. parmentieri'' isolated in two growing seasons under temperate climate conditions and, hence, illustrates high heterogeneity within this species.
|AbstractCC=Yes
|Research topic=molecular biology - genes
|Is book=No
}}
{{Pest record
|Pest=Pectobacterium parmentieri
|Crop=Potato (Solanum tuberosum)
|Country=Poland
|Quarantined=No
}}