View source for Plant Disease (2012) 96, 865-874

{{Publication
|Publication authors=Carla M. Maleita, Maria José Simões, Conceição Egas, [[Rosane H.C. Curtis]] and [[Isabel M. de O. Abrantes]]
|Author Page=Isabel M. de O. Abrantes, Rosane H.C. Curtis
|Publication date=2012
|dc:title=Biometrical, biochemical, and molecular diagnosis of Portuguese ''[[Meloidogyne hispanica]]'' isolates
|Publication journal=Plant Disease
|prism:volume=96 (6)
|prism:startingPage=865
|Last page=874
|DOI=10.1094/PDIS-09-11-0769-RE
|Publication abstract=''Meloidogyne hispanica'' infects many economically important crops worldwide. The accurate identification of this pathogen is essential for the establishment of efficient and sustainable integrated pest management programs. Portuguese ''M. hispanica'' isolates were studied by biometrical, biochemical, and molecular characteristics. Biometrical characteristics of ''M. hispanica'' females, males, and second-stage juveniles were similar to the original description. Biochemical studies revealed a unique enzyme pattern (Hi4) for ''M. hispanica'' esterases that allowed for species differentiation. Molecular analysis of the mtDNA region from ''COII'' and 16S rRNA genes resulted in amplification products (1,800 bp) similar to ''M. hispanica, M. ethiopica'', and ''M. javanica'', and the described ''Hin''fI was unable to discriminate ''M. hispanica'' from the other two species. Analysis of the mtDNA sequences revealed altered nucleotides among the isolates that created new restriction sites for ''Alu''I and ''Dra''III. The resulting restriction patterns successfully discriminated between the three species, providing a new tool for ''Meloidogyne'' identification. Finally, the phylogenetic relationship between ''M. hispanica'' and several ''Meloidogyne'' spp. sequences was analyzed using mtDNA, confirming the divergence between meiotic and mitotic species and revealing the proximity of ''M. hispanica'' to closely related species. Based on the studies conducted, the application of isozyme or polymerase chain reaction restriction fragment length polymorphism analysis would be a useful and efficient methodology for ''M. hispanica'' identification.
|AbstractCC=Yes
|Research topic=identification/taxonomy
|Is book=No
}}
{{Pest record
|Pest=Meloidogyne hispanica
|Country=Portugal (continental)
|Quarantined=No
}}