View source for Pest Management Science (2008) 64, 1229-1236

{{Publication
|Publication authors=[[Qin Yu]], Heping Han and [[Stephen B. Powles]]
|Author Page=Stephen B. Powles, Qin Yu
|Publication date=2008
|dc:title=Mutations of the ALS gene endowing resistance to ALS-inhibiting herbicides in ''[[Lolium rigidum (weed)|Lolium rigidum]]'' populations
|Publication journal=Pest Management Science
|prism:volume=64 (12)
|prism:startingPage=1229
|Last page=1236
|DOI=10.1002/ps.1624
|Publication abstract='''BACKGROUND:''' In the important grass weed ''Lolium rigidum'' (Gaud.), resistance to ALS-inhibiting herbicides has evolved widely in Australia. The authors have previously characterised the biochemical basis of ALS herbicide resistance in a number of ''L. rigidum'' biotypes and established that resistance can be due to a resistant ALS and/or enhanced herbicide metabolism. The purpose of this study was to identify specific resistance-endowing ALS gene mutation(s) in four resistant populations and to develop PCR-based molecular markers.<br/>'''RESULTS:''' Six resistance-conferring ALS mutations were identified: Pro-197-Ala, Pro-197-Arg, Pro-197-Gln, Pro-197-Leu, Pro-197-Ser and Trp-574-Leu. All six mutations were found in one population (WLR1). Each Pro-197 mutation conferred resistance to the sulfonylurea (SU) herbicide sulfometuron, whereas the Trp-574-Leu mutation conferred resistance to both sulfometuron and the imidazolinone (IMS) herbicide imazapyr. A derived cleaved amplified polymorphic sequences (dCAPS) marker was developed for detecting resistance mutations at Pro-197. Furthermore, cleaved amplified polymorphic sequences (CAPS) markers were developed for detecting each of the six mutant resistant alleles. Using these markers, the authors revealed diverse ALS-resistant alleles and genotypes in these populations and related them directly to phenotypic resistance to ALS-inhibiting herbicides.<br/>'''CONCLUSION:''' This study established the existence of a diversity of ALS gene mutations endowing resistance in ''L. rigidum'' populations: 1-6 different mutations were found within single populations. At field herbicide rates, resistance profiles were determined more by the specific mutation than by whether plants were homo- or heterozygous for the mutation.
|AbstractCC=Yes
|Research topic=pesticide resistance of pest
|Is book=No
}}
{{Pest record
|Pest=Lolium rigidum (weed)
|Quarantined=No
}}