View source for Pest Management Science (2003) 59, 1143-1151

{{Publication
|Publication authors=[[Guido Schnabel]], Qun Dai and Manjiri R. Paradkar
|Author Page=Guido Schnabel
|Publication date=2003
|dc:title=Cloning and expression analysis of the ATP-binding cassette transporter gene ''MFABC1'' and the alternative oxidase gene ''MfAOX1'' from ''[[Monilinia fructicola]] ''
|Publication journal=Pest Management Science
|prism:volume=59 (10)
|prism:startingPage=1143
|Last page=1151
|DOI=10.1002/ps.744
|Publication abstract=Brown rot, caused by ''Monilinia fructicola'' (G Wint) Honey, is a serious disease of peach in all commercial peach production areas in the USA, including South Carolina where it has been primarily controlled by pre-harvest application of 14- demethylation (DMI) fungicides for more than 15 years. Recently, the Qo fungicide azoxystrobin was registered for brown rot control and is currently being investigated for its potential as a DMI fungicide rotation partner because of its different mode of action. In an effort to investigate molecular mechanisms of DMI and Qo fungicide resistance in ''M. fructicola'', the ABC transporter gene ''MfABC1'' and the alternative oxidase gene ''MfAOX1'' were cloned to study their potential role in conferring fungicide resistance. The ''MfABC1'' gene was 4380 bp in length and contained one intron of 71 bp. The gene revealed high amino acid homologies with atrB from ''Aspergillus nidulans'' (Eidam) Winter, an ABC transporter conferring resistance to many fungicides, including DMI fungicides. ''MfABC1'' gene expression was induced after myclobutanil and propiconazole treatment in isolates with low sensitivity to the same fungicides, and in an isolate with high sensitivity to propiconazole. The results suggest that the ''MfABC1'' gene may be a DMI fungicide resistance determinant in ''M. fructicola.<br/>''The alternative oxidase gene ''MfAOX1'' from ''M. fructicola'' was cloned and gene expression was analyzed. The ''MfAOX1'' gene was 1077 bp in length and contained two introns of 54 and 67 bp. The amino acid sequence was 63.8, 63.8 and 57.7% identical to alternative oxidases from ''Venturia inaequalis'' (Cooke) Winter, ''Aspergillus niger'' van Teighem and ''A. nidulans'', respectively. ''MfAOX1'' expression in some but not all ''M fructicola'' isolates was induced in mycelia treated with azoxystrobin. Azoxystrobin at 2 µg ml<sup>-1</sup> significantly induced ''MfAOX1'' expression in isolates with low ''MfAOX1'' constitutive expression levels.
|AbstractCC=Yes
|Research topic=control - general
|Is book=No
}}
{{Pest record
|Pest=Monilinia fructicola
|Crop=Peach/nectarine (Prunus persica)
|Country=U.S.A. (SE)
|Quarantined=No
}}