View source for New Phytologist (2009) 181, 435-447

{{Publication
|Publication authors=M. Barret, P. Frey-Klett, M. Boutin, A.-Y. Guillerm-Erckelboudt, F. Martin, L. Guillot and [[Alain Sarniguet|A. Sarniguet]]
|Author Page=Alain Sarniguet
|Publication date=2009
|dc:title=The plant pathogenic fungus ''[[Gaeumannomyces tritici|Gaeumannomyces graminis]]'' var. ''tritici'' improves bacterial growth and triggers early gene regulations in the biocontrol strain ''[[Pseudomonas fluorescens (antagonist)|Pseudomonas fluorescens]]'' Pf29Arp
|Publication journal=New Phytologist
|prism:volume=181 (2)
|prism:startingPage=435
|Last page=447
|DOI=10.1111/j.1469-8137.2008.02675.x
|Publication abstract=- In soil, some antagonistic rhizobacteria contribute to reduce root diseases caused by phytopathogenic fungi. Direct modes of action of these bacteria have been largely explored; however, commensal interaction also takes place between these microorganisms and little is known about the influence of filamentous fungi on bacteria. <br/>- An ''in vitro'' confrontation bioassay between the pathogenic fungus ''Gaeumannomyces graminis ''var.'' tritici'' (''Ggt'') and the biocontrol bacterial strain ''Pseudomonas fluorescens'' Pf29Arp was set up to analyse bacterial transcriptional changes induced by the fungal mycelium at three time-points of the interaction before cell contact and up until contact. For this, a Pf29Arp shotgun DNA microarray was constructed. Specifity of ''Ggt'' effect was assessed in comparison with one of two other filamentous fungi, ''Laccaria bicolor'' and ''Magnaporthe grisea''. <br/>- During a commensal interaction,'' Ggt'' increased the growth rate of Pf29Arp. Before contact, ''Ggt'' induced bacterial genes involved in mycelium colonization. At contact, genes encoding protein of stress response and a patatin-like protein were up-regulated. Among all the bacterial genes identified, ''xseB'' was specifically up-regulated at contact by ''Ggt'' but down-regulated by the other fungi. <br/>- Data showed that the bacterium sensed the presence of the fungus early, but the main gene alteration occurred during bacterial-fungal cell contact.
|AbstractCC=Yes
|Research topic=biocontrol - natural enemies
|Benf research=general biology - morphology - evolution
|Research2B=molecular biology - genes
|Is book=No
}}
{{Pest record
|Pest=Gaeumannomyces tritici
|Crop=Wheat (Triticum)
|Quarantined=No
}}
{{Beneficial record
|Beneficial=Pseudomonas fluorescens (antagonist)
|Pest=Gaeumannomyces tritici
|Crop=Wheat (Triticum)
}}