View source for Molecular Ecology Notes (2007) 7, 947-954

{{Publication
|Publication authors=[[Gui-Fen Zhang]], Zhi-Chuang Lü, [[Fang Hao Wan|Fang-Hao Wan]] and [[Gábor L. Lövei]]
|Author Page=Fang Hao Wan, Gábor L. Lövei, Gui-Fen Zhang
|Publication date=2007
|dc:title=Real-time PCR quantification of ''Bemisia tabaci'' (Homoptera: Aleyrodidae) B-biotype remains in predator guts
|Publication journal=Molecular Ecology Notes
|prism:volume=7 (6)
|prism:startingPage=947
|Last page=954
|DOI=10.1111/j.1471-8286.2007.01819.x
|Publication abstract=The cotton whitefly, ''Bemisia tabaci'' (Gennadius) B-biotype, is fed on by a wide variety of generalist predators, but there is little information on these predator-prey interactions, especially under field conditions. In this study, a real-time polymerase chain reaction (PCR) assay was developed to quantify ''B. tabaci'' B-biotype remains in predator gut. The ''B. tabaci'' B-biotype genomic DNA copy number was referred to the actual amount of BT1 isolate, the ''B. tabaci'' B-biotype specific DNA fragment. The numbers of BT1 isolate in one ''B. tabaci'' B-biotype egg, individual adult and a single red-eyed nymph were 2.56 × 10<sup>3</sup>, 2.56 × 10<sup>4</sup>, and 1.29 × 10<sup>4</sup> copies, respectively. When ''Propylaea japonica'' adults fed on one, two, four, eight or 16 red-eyed nymphs, the detected numbers of BT1 isolate ranged from 2.77 × 10<sup>4</sup> to 4.05 × 10<sup>5</sup> copies, forming a strong linear relationship (R<sup>2</sup> = 0.9899). Following the consumption of two red-eyed nymphs, prey DNA was detectable in 100% of ''P. japonica'' at t = 0, decreasing to 80.0% and 60.0% after 1-4 h and 8 h of digestion, respectively, with 3.36 × 10<sup>4</sup>-1.25 × 10<sup>3</sup> BT1 isolate copies. The predation by field-collected predators, 26 larvae of ''P. japonica'', and of ''Harmonia axyridis'' each, ''Chrysopa'' spp. larvae (''Chrysopa pallens'' and ''C. formosa'', 18 individuals in total), and a single adult of ''Scymnus hoffmanni'', 19 adults of ''Orius sauteri'' and nine adult spiders (''Erigonnidium graminicolum'' and ''Neoscona doenitzi''), on ''B. tabaci'' B-biotype were quantified. Of the 99 analysed predator individuals, 3.65 × 10<sup>2</sup> - 4.60 × 10<sup>5</sup> copies of BT1 isolate, equivalent to 0.8-18.8 red-eyed nymphs were detected. These results suggest that TaqMan real-time PCR technology may provide a rapid and sensitive method for quantifying ''B. tabaci'' B-biotype remains in predator guts and will be invaluable in assessing the food web relationship between prey and arthropod predators.
|AbstractCC=Yes
|Research topic=biocontrol - natural enemies
|Benf research=general biology - morphology - evolution
|Is book=No
}}
{{Pest record
|Pest=Bemisia tabaci biotype MEAM1
|Quarantined=No
}}
{{Beneficial record
|Beneficial=Harmonia axyridis (predator)
|Pest=Bemisia tabaci biotype MEAM1
}}
{{Beneficial record
|Beneficial=Propylea japonica (predator)
|Pest=Bemisia tabaci biotype MEAM1
}}
{{Beneficial record
|Beneficial=Scymnus hoffmanni (predator)
|Pest=Bemisia tabaci biotype MEAM1
}}
{{Beneficial record
|Beneficial=Chrysopa pallens (predator)
|Pest=Bemisia tabaci biotype MEAM1
}}
{{Beneficial record
|Beneficial=Orius sauteri (predator)
|Pest=Bemisia tabaci biotype MEAM1
}}
{{Beneficial record
|Beneficial=Chrysopa formosa (predator)
|Pest=Bemisia tabaci biotype MEAM1
}}
{{Beneficial record
|Beneficial=Neoscona doenitzi (predator)
|Pest=Bemisia tabaci biotype MEAM1
}}