View source for Letters in Applied Microbiology (2001) 32, 166-170

{{Publication
|Publication authors=J. Lydon and C.D. Patterson
|Publication date=2001
|dc:title=Detection of tabtoxin-producing strains of ''[[Pseudomonas syringae]]'' by PCR
|Publication journal=Letters in Applied Microbiology
|prism:volume=32 (3)
|prism:startingPage=166
|Last page=170
|DOI=10.1046/j.1472-765x.2001.00882.x
|Publication abstract='''Aims:''' The present study describes a system based on PCR to distinguish tabtoxin-producing strains of ''Pseudomonas syringae'' from other ''Ps. syringae'' plant pathogens that produce chlorosis-inducing phytotoxins. <br/>'''Methods and Results:''' Thirty-two strains of ''Ps. syringae'' and related species were examined. Two sets of PCR primers were developed to amplify genes (''tblA'' and ''tabA'') required for tabtoxin production. Only a PCR product of 829 bp or 1020 bp was produced in PCR reactions with the tblA or tabA primer sets, respectively, and cells from tabtoxin-producing pathovars of ''Pseudomonas syringae''. All known non-tabtoxin producing bacterial species failed to produce an amplification product with either primer set. <br/>'''Conclusions:''' PCR of genes required for tabtoxin production is a simple, rapid and reliable method for identifying tabtoxin-producing strains of ''Ps. syringae''. <br/>'''Significance and Impact of the Study:''' The protocol can effectively distinguish tabtoxin-producing strains of ''Ps. syringae'' from other ''Ps. syringae'' pathovars and ''Ps. syringae'' pv. ''tabaci'' strains from other tabtoxin-producing ''Ps. syringae'' pathovars.
|AbstractCC=Yes
|Research topic=identification/taxonomy
|Is book=No
}}
{{Pest record
|Pest=Pseudomonas syringae
|Quarantined=No
}}
{{Pest record
|Pest=Pseudomonas syringae pv. tabaci
|Quarantined=No
}}