View source for Journal of Phytopathology (2014) 162, 147-157

{{Publication
|Publication authors=Jian-Hua Wang, Jing-Bo Zhang, He-Ping Li, An-Dong Gong, Sheng Xue, Rebecca S. Agboola and [[Yu-Cai Liao]]
|Author Page=Yu-Cai Liao
|Publication date=2014
|dc:title=Molecular identification, mycotoxin production and comparative pathogenicity of ''[[Fusarium temperatum]]'' isolated from maize in China
|Publication journal=Journal of Phytopathology
|prism:volume=162 (3)
|prism:startingPage=147
|Last page=157
|DOI=10.1111/jph.12164
|Publication abstract=A recently isolated ''Fusarium'' population from maize in Belgium was identified as a new species, ''Fusarium temperatum''. From a survey of ''Fusarium'' species associated with maize ear rot in nineteen provinces in 2009 in China, ten strains isolated from Guizhou and Hubei provinces were identified as ''F. temperatum''. Morphological and molecular phylogenetic analyses based on the DNA sequences of individual translation elongation factor 1-alpha and β-tubulin genes revealed that the recovered isolates produced macroconidia typical of four-septate with a foot-shaped basal cell and belonged to ''F. temperatum'' that is distinctly different from its most closely related species ''F. subglutinans'' and others within ''Gibberella fujikuroi'' complex species from maize. All the strains from this newly isolated species were able to infect maize and wheat in field, with higher pathogenicity on maize. Mycotoxin determination of maize grains infected by the strains under natural field condition by ultra-high-performance liquid chromatography-tandem mass spectrometry and gas chromatography-mass spectrometry analyses showed that among fifteen mycotoxins assayed, two mycotoxins fumonisin B<sub>1</sub> and B<sub>2</sub> ranging from 9.26 to 166.89 ''μ''g/g were detected, with massively more FB<sub>2</sub> mycotoxin (2.8- to 108.8-fold) than FB<sub>1</sub>. This mycotoxin production profile is different from that of the Belgian population in which only fumonisin B<sub>1</sub> was barely detected in one of eleven strains assayed. Comparative analyses of the ''F''. ''temperatum'' and ''F. subglutinans'' strains showed that the highest fumonisin producers were present among the ''F''. ''temperatum'' population, which were also the most pathogenic to maize. These results suggested a need for proper monitoring and controlling this species in the relevant maize-growing regions.
|AbstractCC=Yes
|Research topic=surveys/sampling/distribution
|Research2P=general biology - morphology - evolution
|Is book=No
}}
{{Pest record
|Pest=Fusarium temperatum
|Crop=Maize/corn (Zea mays)
|Country=China (south)
|Quarantined=No
}}