View source for FEMS Microbiology Letters (2009) 291, 127-135

{{Publication
|Publication authors=Min He and [[Yuxian Xia]]
|Author Page=Yuxian Xia
|Publication date=2009
|dc:title=Construction and analysis of a normalized cDNA library from ''Metarhizium anisopliae'' var. ''acridum'' germinating and differentiating on ''[[Locusta migratoria]]'' wings
|Publication journal=FEMS Microbiology Letters
|prism:volume=291 (1)
|prism:startingPage=127
|Last page=135
|DOI=10.1111/j.1574-6968.2008.01447.x
|Publication abstract=A cDNA library construction method was established that allows gene expression of the entomopathogenic fungus ''Metarhizium anisopliae'' var. ''acridum'' to be studied during its growth on ''Locusta migratoria'' wings. Before inoculation, locust wings were treated with an RNA fragmentation buffer to degrade host nucleic acid. Reverse transcription-PCR analysis of several constitutively expressed locust genes was used to confirm the degradation of locust nucleic acid. A normalized cDNA library of ''M. anisopliae'' var. ''acridum'' germinating and differentiating on locust wings was constructed and analyzed. Analysis of 162 unigenes out of 221 sequenced inserts from the cDNA library revealed that the cDNA library was not contaminated with locust transcripts, and contained previously characterized genes known to be expressed during infection. Further studies of the expressed sequence tags from this library will provide insights into the molecular basis of infection of this fungus.
|AbstractCC=Yes
|Research topic=biocontrol - natural enemies
|Benf research=molecular biology - genes
|Is book=No
}}
{{Pest record
|Pest=Locusta migratoria
|Quarantined=No
}}
{{Beneficial record
|Beneficial=Metarhizium acridum (entomopathogen)
|Pest=Locusta migratoria
}}