View source for FEMS Microbiology Letters (2005) 243, 505-511

{{Publication
|Publication authors=He-Ping Li, Ai-Bo Wu, Chun-Sen Zhao, [[Olga E. Scholten|Olga Scholten]], Huub Löffler and [[Yu-Cai Liao]]
|Author Page=Yu-Cai Liao, Olga E. Scholten
|Publication date=2005
|dc:title=Development of a generic PCR detection of deoxynivalenol- and nivalenol-chemotypes of ''[[Fusarium graminearum]] ''
|Publication journal=FEMS Microbiology Letters
|prism:volume=243 (2)
|prism:startingPage=505
|Last page=511
|DOI=10.1016/j.femsle.2005.01.015
|Publication abstract=Based on the intergenic sequences of ''Tri5-Tri6'' genes involved in the mycotoxin pathways of ''Fusarium'' species, a generic PCR assay was developed to detect a 300 bp fragment of deoxynivalenol (DON)-chemotypes and a 360 bp sequence of nivalenol (NIV)- chemotypes of ''Fusarium graminearum''. Mycotoxin chemotypes identified by the PCR assays were confirmed by the chemical analyses of HPLC or GC/MS. Further analysis of 364 ''F. graminearum'' isolates from 12 provinces of China showed that 310 were DON-chemotypes and 54 were NIV-chemotypes. Sequence analyses revealed that DON-chemotypes display more variations than NIV-chemotypes. This PCR assay could be used to detect mycotoxin-producing ''Fusarium''-species and may thus help to develop strategies to avoid or reduce mycotoxin contamination of cereals. Also this assay may provide useful alternatives to antibody-based mycotoxin tests.
|AbstractCC=Yes
|Research topic=molecular biology - genes
|Is book=No
}}
{{Pest record
|Pest=Fusarium graminearum
|Quarantined=No
}}