View source for Applied and Environmental Microbiology (2005) 71, 1169-1177

{{Publication
|Publication authors=Olivier Vandeputte, Sevgi Öden, Adeline Mol, Danny Vereecke, [[Koen Goethals]], [[Mondher Jaziri|Mondher El Jaziri]] and Els Prinsen
|Author Page=Mondher Jaziri, Koen Goethals
|Publication date=2005
|dc:title=Biosynthesis of auxin by the gram-positive phytopathogen ''[[Rhodococcus fascians]]'' is controlled by compounds specific to infected plant tissues
|Publication journal=Applied and Environmental Microbiology
|prism:volume=71 (3)
|prism:startingPage=1169
|Last page=1177
|DOI=10.1128/AEM.71.3.1169-1177.2005
|Publication abstract=The role and metabolism of indole-3-acetic acid in gram-negative bacteria is well documented, but little is known about indole-3-acetic acid biosynthesis and regulation in gram-positive bacteria. The phytopathogen ''Rhodococcus fascians'', a gram-positive organism, incites diverse developmental alterations, such as leafy galls, on a wide range of plants. Phenotypic analysis of a leafy gall suggests that auxin may play an important role in the development of the symptoms. We show here for the first time that ''R. fascians<sup>'' </sup>produces and secretes the auxin indole-3-acetic acid. Interestingly, whereas noninfected-tobacco extracts have no effect, indole-3-acetic acid synthesis is highly induced in the presence of infected-tobacco extracts when tryptophan is not limiting. Indole-3-acetic acid production by a plasmid-free strain shows that the biosynthetic genes are located on the bacterial chromosome, although plasmid-encoded genes contribute to the kinetics and regulation of indole-3-acetic acid biosynthesis. The indole-3-acetic acid intermediates present in bacterial cells and secreted into the growth media show that the main biosynthetic route used by ''R. fascians'' is the indole-3-pyruvic acid pathway with a possible rate-limiting role for indole-3-ethanol. The relationship between indole-3-acetic acid production and the symptoms induced by ''R. fascians'' is discussed.
|AbstractCC=Yes
|Research topic=molecular biology - genes
|Is book=No
}}
{{Pest record
|Pest=Rhodococcus fascians
|Quarantined=No
}}