Plant Pathology (2007) 56, 332-336

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M. Nicolaisen and A. Bertaccini (2007)
An oligonucleotide microarray-based assay for identification of phytoplasma 16S ribosomal groups
Plant Pathology 56 (2), 332-336
Abstract: A method using consensus PCR followed by oligonucleotide microarray hybridization was developed for identification of phytoplasma 16Sr ribosomal groups. The array consisted of 21- to 33-nt-long oligonucleotides which were designed to hybridize to individual 16Sr groups. Two oligonucleotides were designed to detect all phytoplasma groups. The array could efficiently identify samples from 16SrI, 16SrII, 16SrIII, 16SrV, 16SrVI, 16SrVII, 16SrIX, 16SrX and 16SrXII ribosomal groups. This microarray-based test represents a rapid method for detection of phytoplasmas in unknown samples and for identification of most 16Sr groups.
(The abstract is excluded from the Creative Commons licence and has been copied with permission by the publisher.)
Link to article at publishers website
Database assignments for author(s): M. Nicolaisen, Assunta Bertaccini

Research topic(s) for pests/diseases/weeds:
identification/taxonomy
molecular biology - genes


Pest and/or beneficial records:

Beneficial Pest/Disease/Weed Crop/Product Country Quarant.


16SrI phytoplasma group
16SrII phytoplasma group
16SrX phytoplasma group
16SrXII phytoplasma group
16SrVII phytoplasma group
16SrV phytoplasma group
16SrVI phytoplasma group
16SrIII phytoplasma group