Plant Breeding (2011) 130, 16-24

N. Meyer, V. Lind, P. Karlovsky, M. Zahn, W. Friedt and F. Ordon (2011)
Development of a real-time PCR method for the identification of wheat genotypes carrying different eyespot resistance genes
Plant Breeding 130 (1), 16-24
Abstract: Eyespot, caused by Oculimacula acuformis and O. yallundae, is an important disease of wheat (Triticum aestivum). The two pathogens generate similar symptoms and cannot be distinguished visually. In order to distinguish O. acuformis and O. yallundae infections and to identify reliable quantitative differences in the level of resistance of wheat genotypes, two SYBR Green based real-time PCR assays were developed. The influence of time after inoculation until sample collection, the effect of species (O. acuformis, O. yallundae), and the relevance of wheat cultivars carrying Pch1, Pch2, or none of the resistance genes on the pathogen-DNA content were analysed. The most clear-cut difference of pathogen content between resistant and susceptible wheat genotypes was obtained 12 weeks after inoculation with O. yallundae. Growth chamber tests using artificial inoculation with O. yallundae and sampling 12 weeks after inoculation for real-time PCR is shown to be a reliable procedure to determine the resistance level of wheat against eyespot. Likewise, this is the basis for the development of efficient molecular markers.
(The abstract is excluded from the Creative Commons licence and has been copied with permission by the publisher.)
Link to article at publishers website
Database assignments for author(s): Petr Karlovsky, Frank Ordon

Research topic(s) for pests/diseases/weeds:
resistance/tolerance/defence of host

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Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
	Oculimacula yallundae	Wheat (Triticum)
	Oculimacula acuformis	Wheat (Triticum)