Pesquisa Agropecuária Brasileira (2008) 43, 513-520
Andréa Almeida Carneiro, Eliane Aparecida Gomes, Claudia Teixeira Guimarães, Fernando Tavares Fernandes, Newton Portilho Carneiro and Ivan Cruz (2008)
Molecular characterization and pathogenicity of isolates of Beauveria spp. to fall armyworm
Pesquisa Agropecuária Brasileira 43 (4), 513-520
Abstract: The objective of this work was to evaluate the pathogenicity of 24 Beauveria isolates to Spodoptera frugiperda larvae, and characterize them molecularly through rDNA-ITS sequencing and RAPD markers. Sequencing of rDNA-ITS fragments of 570 bp allowed the identification of isolates as B. bassiana or B. brongniarti by sequence comparison to GenBank. Sixty seven polymorphic RAPD fragments were capable to differentiate 20 among 24 Beauveria isolates, grouping them according to the derived host insect and to pathogenicity against maize fall armyworm larvae. Three RAPD markers were highly associated to the pathogenicity against S. frugiperda, explaining up to 67% of the phenotypic variation. Besides identification and molecular characterization of Beauveria isolates, ITS sequence and RAPD markers proved to be very useful in selecting the isolates potentially effective against S. frugiperda larvae and in monitoring field release of these microorganisms in biocontrol programs.
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Database assignments for author(s): Claudia Teixeira Guimarães, Ivan Cruz
Research topic(s) for pests/diseases/weeds:
biocontrol - natural enemies
Research topic(s) for beneficials or antagonists:
surveys/distribution/isolation
molecular biology - genes
