Molecular Breeding (1998) 4, 501-507
Shahina Bano Maqbool, T. Husnain, S. Riazuddin, L. Masson and P. Christou (1998)
Effective control of yellow stem borer and rice leaf folder in transgenic rice indica varieties Basmati 370 and M 7 using the novel delta-endotoxin cry2A Bacillus thuringiensis gene
Molecular Breeding 4 (6), 501-507
Abstract: Transgenic rice indica varieties Basmati 370 and M 7 expressing the novel cry2A (Bt) insecticidal gene were generated by particle bombardment. Molecular and biochemical analyses in R0 and R1 populations confirmed stable integration and expression of this novel Bt transgene. We estimated that the gene product was expressed up to 5% of total leaf protein. Insect feeding bioassays demonstrated that the Cry2A protein was effective against the yellow stem borer and the rice leaf folder, two major rice pests in the Indian Subcontinent. This is the first report of the control of major rice pests using this specific Bt gene. The cry2A gene can now be used in combination with other insecticidal genes for pyramiding resistance against insect pests. This will delay, or perhaps in combination with integrated pest management practices, prevent evolution of insect populations resistant to single insecticidal genes.
(The abstract is excluded from the Creative Commons licence and has been copied with permission by the publisher.)
Link to article at publishers website
Database assignments for author(s): Tayyab Husnain, Paul Christou, Luke Masson
Research topic(s) for pests/diseases/weeds:
biocontrol - natural enemies
resistance/tolerance/defence of host
Research topic(s) for beneficials or antagonists:
genetical engin./transgenic plants
