Medical and Veterinary Entomology (2007) 21, 30-35

C. Walton, P. Somboon, R.E. Harbach, S. Zhang, I. Weerasinghe, S.M. O'Loughlin, S. Phompida, T. Sochantha, W. Tun-Lin, B. Chen and R.K. Butlin (2007)
Molecular identification of mosquito species in the Anopheles annularis group in southern Asia
Medical and Veterinary Entomology 21 (1), 30-35
Abstract: The Anopheles annularis group of subgenus Cellia Theobald (Diptera: Culicidae) includes five currently recognized species in southern Asia: An. annularis Van der Wulp, Anopheles nivipes (Theobald) and Anopheles philippinensis Ludlow, which are widespread in the region, Anopheles pallidus Theobald, which is known in Sri Lanka, India and Myanmar, and Anopheles schueffneri Stanton, which occurs in Java and Sumatra. Identification of the four mainland species based on morphology is problematic. In view of the fact that the three widespread species are variously involved in malaria transmission in different parts of the region, we developed a species-specific polymerase chain reaction assay based on rDNA internal transcribed spacer 2 (ITS2) sequences to facilitate entomological and epidemiological studies of the four species. The method proved to be reliable when tested over a wide geographical area.
(The abstract is excluded from the Creative Commons licence and has been copied with permission by the publisher.)
Link to article at publishers website
Database assignments for author(s): Pradya Somboon, Ralph E. Harbach

Research topic(s) for pests/diseases/weeds:
identification/taxonomy
molecular biology - genes

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Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
	Anopheles annularis
	Anopheles nivipes
	Anopheles philippinensis
	Anopheles pallidus		Myanmar
	Anopheles pallidus		Sri Lanka
	Anopheles pallidus		India