Journal of Plant Pathology (2005) 87, 141-144

M. Guardo, T. Marletta, G. Boninelli, A. Leonardi, G. Sorrentino and A. Caruso (2005)
Detection of viroids in forced citrus cuttings
Journal of Plant Pathology 87 (2), 141-144
Abstract: A procedure was developed for detecting viroids in citrus cuttings collected in the field and forced to sprout in a growth chamber. Leaves collected 15 days or one month after sprouting were tested in comparison with leaf and bark samples from field-grown source plants. Tests were made by using RT-PCR with primers specific for Citrus exocortis viroid (CEVd), the citrus strain of Hop stunt viroid (HpSVd-cit), Citrus bent leaf viroid (CBLVd), Citrus viroid III (CVd-III), or Citrus viroid IV (CVd-IV). No evidence was obtained for the presence of CBLVd and CVd-IV. However, CEVd, and CVd-III were detected consistently in forced cuttings even when no positive amplification was obtained from field samples. This method may be a useful in the laboratory detection of citrus viroids.
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Database assignments for author(s): Maria Guardo

Research topic(s) for pests/diseases/weeds:
surveys/sampling/distribution

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Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
	Citrus exocortis viroid	Citrus (genus)	Italy
	Hop stunt viroid	Citrus (genus)
	Citrus dwarfing viroid	Citrus (genus)	Italy
	Citrus bent leaf viroid	Citrus (genus)
	Citrus bark cracking viroid	Citrus (genus)