Journal of Plant Pathology (2004) 86, 317-318
A. Fabi, M. Pistola, M. Antonelli and L. Varvaro (2004)
In vitro evaluation of antimicrobial activity of low copper compounds against tomato phytopathogenic bacteria and their in vivo efficacy on Xanthomonas vesicatoria
Journal of Plant Pathology 86 (4), 317-318
XI Meeting, Italian Society for Plant Pathology, Milan, 29/9 - 1/10, 2004 (poster)
Abstract: In vitro studies have been carried out in order to evaluate the bactericidal activity of low copper compounds (pentahydrate sulphate and tribasic sulphate) against several strains of Clavibacter michiganensis subsp. michiganensis (Cmm), Pseudomonas syringae pv. tomato (Pst), Xanthomonas vesicatoria (Xv), Pseudomonas corrugata (Pc), and Ralstonia solanacearum (Rs). Different methods were used: i) wells were made in agar plates inoculated with bacterial strains, then filled with different concentrations of the two tested substances; the inhibition haloes of bacterial growth were measured around each well; ii) bacterial growth on agar media containing different amounts of these substances was determined; iii) the multiplication of bacteria in '523' broth containing different concentrations of copper compounds was assessed, as well as iv) the survival of bacteria in solutions of sterile distilled water and copper compounds. In vivo studies were carried out on susceptible tomato (Lycopersicon esculentum L.) plants. These were sprayed with suspensions of Xv after or before treatments with copper pentahydrate sulphate, with the addition or not of a solution of betaine and lecithin (Betasan®) as adhesive. Epiphytic bacterial population of Xv was then evaluated by leaf washing and plating, up to 15 days after inoculation. The anti-microbial activity of copper pentahydrate sulphate against bacterial strains was found to be higher than that of tribasic sulphate in all the four tests made, especially in broth and in SDW where no bacteria were detected after 12 h with the highest copper concentration. A strain of Xv resulted particularly sensitive, especially when using the well method. The in vivo experiments showed a very low bacterial multiplication on plant leaves treated with copper before inoculation (especially on plants treated with copper three days before) if compared with multiplication on plants treated after inoculation or non-treated. No particular effect of adhesive Betasan® was detected.
(The abstract is excluded from the Creative Commons licence and has been copied with permission by the publisher.)
Database assignments for author(s): Leonardo Varvaro
Research topic(s) for pests/diseases/weeds:
control - general
