Journal of Economic Entomology (2007) 100, 164-171

Fangneng Huang, B. Rogers Leonard and David A. Andow (2007)
Sugarcane borer (Lepidoptera: Crambidae) resistance to transgenic Bacillus thuringiensis maize
Journal of Economic Entomology 100 (1), 164-171
Abstract: Transgenic maize, Zea mays L., expressing the Bacillus thuringiensis (Bt) Cry1Ab toxin has been planted to extensive areas across the United States and several other countries, but no resistance has been documented in field populations of lepidopteran target pests. This article describes the first report of resistance alleles to commercially available Cry1Ab Bt maize in a Louisiana population of sugarcane borer, Diatraea saccharalis (F.) (Lepidoptera: Crambidae). Two hundred thirteen two-parent isolines of D. saccharalis were screened for Cry1Ab resistance on Bt maize leaf tissue using an F₂ screening technique. Larvae representing three isolines survived >15 d on Bt tissue in the F₂ generation. The second generation backcross progeny (B₁F₂) derived from isoline 52 completed larval development on Bt maize in the greenhouse. Segregation and resistance frequency analysis associated with isoline 52 suggested that Bt resistance is probably determined by a nearly completely recessive allele at a single locus. With this assumption, the estimated resistance allele frequency in this population is 0.0023, within a 95% confidence interval of 0.0003-0.0064.
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Database assignments for author(s): Fang Neng Huang

Research topic(s) for pests/diseases/weeds:
biocontrol - natural enemies
Research topic(s) for beneficials or antagonists:
resistance/tolerance/defence of host

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Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
	Diatraea saccharalis	Maize/corn (Zea mays)	U.S.A. (mid S)
Bacillus thuringiensis genes in crops (entomopathogen)	Diatraea saccharalis	Maize/corn (Zea mays)	U.S.A. (mid S)
Bacillus thuringiensis Cry1A-toxin (entomopathogen)	Diatraea saccharalis	Maize/corn (Zea mays)	U.S.A. (mid S)