Journal of Chemical Ecology (2012) 38, 1521-1527

C. Wise, L. Novitsky, A. Tsopmo and T.J. Avis (2012)
Production and antimicrobial activity of 3-hydroxypropionaldehyde from Bacillus subtilis strain CU12
Journal of Chemical Ecology 38 (12), 1521-1527
Abstract: Bacillus subtilis strains are known to produce a vast array of antimicrobial compounds. However, some compounds remain to be identified. Disk assays performed in vitro with Bacillus subtilis CU12 showed a significant reduction in mycelial growth of Alternaria solani, Botrytis cinerea, Fusarium sambucinum, and Pythium sulcatum. Crude B. subtilis culture filtrates were subsequently extracted with ethyl acetate and butanol. A bioassay guided purification procedure revealed the presence of one major antifungal compound in the butanol extract. Purification of the compound was performed using a reverse-phase C18 solid phase extraction (SPE) cartridge and flash column chromatography. NMR data showed that the main antimicrobial compound was a cyclic dimer of 3-hydroxypropionaldehyde (HPA). This study demonstrated the antimicrobial activity of B. subtilis strain CU12 against phytopathogenic microorganisms is mediated at least in part by the production of HPA. It also suggests that this B. subtilis strain could be effective at controlling pathogens through protection of its ecological niche by antibiosis.
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Link to article at publishers website

Research topic(s) for pests/diseases/weeds:
biocontrol - natural enemies
Research topic(s) for beneficials or antagonists:
molecular biology - genes

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Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
	Botrytis cinerea
	Pythium sulcatum
	Fusarium sambucinum
	Alternaria solani
Bacillus subtilis (antagonist)	Botrytis cinerea
Bacillus subtilis (antagonist)	Pythium sulcatum
Bacillus subtilis (antagonist)	Fusarium sambucinum
Bacillus subtilis (antagonist)	Alternaria solani