Journal of Applied Entomology (2013) 137, 122-135

L.L. Esterhuizen, K.G. Mabasa, S.W. van Heerden, H. Czosnek, J.K. Brown, H. van Heerden and M.E.C. Rey (2013)
Genetic identification of members of the Bemisia tabaci cryptic species complex from South Africa reveals native and introduced haplotypes
Journal of Applied Entomology 137 (1-2), 122-135
Abstract: The whitefly Bemisia tabaci cryptic species complex contains some important agricultural pest and virus vectors. Members of the complex have become serious pests in South Africa (SA) because of their feeding habit and their ability to transmit begomovirus species. Despite their economic importance, studies on the biology and distribution of B. tabaci in SA are limited. To this end, a survey was made to investigate the diversity and distribution of B. tabaci cryptic species in eight geographical locations (provinces) in SA, between 2002 and 2009, using the mitochondrial cytochrome oxidase I (mtCOI) sequences. Phylogenetic analysis revealed the presence of members from two endemic sub-Saharan Africa (SSAF) subclades coexisting with two introduced putative species. The SSAF-1 subclade includes cassava host-adapted B. tabaci populations, whereas the whiteflies collected from cassava and non-cassava hosts formed a distinct subclade, referred to as SSAF-5, and represent a new subclade among previously recognized southern Africa clades. Two introduced cryptic species, belonging to the Mediterranean and Middle East-Asia minor 1 clades, were identified and include the B and Q types. The B type showed the widest distribution, being present in five of the eight provinces explored in SA, infesting several host plants and predominating over the indigenous haplotypes. This is the first report of the occurrence of the exotic Q type in SA alongside the more widely distributed B type. Furthermore, mtCOI PCR-RFLP was developed for the SA context to allow rapid discrimination between the B, Q and SSAF putative species. The capacity to manage pests and disease effectively relies on knowledge of the identity of the agents causing the damage. Therefore, this study contributes to the understanding of South African B. tabaci species diversity, information needed for the development of knowledge-based disease management practices.
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Link to article at publishers website
Database assignments for author(s): Marie Emma Christine Rey

Research topic(s) for pests/diseases/weeds:
molecular biology - genes
surveys/sampling/distribution

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Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
	Bemisia tabaci biotype MEAM1		South Africa
	Bemisia tabaci biotype MED		South Africa
	Bemisia tabaci biotype Sub Saharan Africa 1	Cassava (Manihot esculenta)	South Africa