European Journal of Plant Pathology (1999) 105, 389-395

F.B. Liu, E. Sukhacheva, T. Erokhina and J. Schubert (1999)
Detection of potyviral nuclear inclusion b proteins by monoclonal antibodies raised to synthetic peptides
European Journal of Plant Pathology 105 (4), 389-395
Abstract: Potyviral nuclear inclusion b protein (NIb), the RNA-dependent RNA polymerase, contains three highly conserved regions. Peptides corresponding to these regions were synthesised and used for immunisation. A panel of monoclonal antibodies was obtained. Most of the MAbs reacted with the peptides and a recombinant NIb of PVY in PTA-ELISA. Two of them specifically detected native NIb of potato A, potato V, potato Y, plum pox and turnip mosaic potyviruses in extracts of infected plants in Western blots. Time course experiments revealed that NIb protein can be first detected on the fifth day after infection.
(The abstract is excluded from the Creative Commons licence and has been copied with permission by the publisher.)
Link to article at publishers website

Research topic(s) for pests/diseases/weeds:
molecular biology - genes

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Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
	Potato virus Y
	Potato virus V
	Plum pox virus
	Potato virus A
	Turnip mosaic virus