European Journal of Entomology (2021) 118, 335-343
Sofia G. Seabra, Joana Martins, Patrícia Brás, Ana M. Tavares, Inês Freitas, António Barata, Maria Teresa Rebelo, Célia Mateus, Octávio S. Paulo and Elisabete Figueiredo (2021)
PCR-based detection of prey DNA in the gut contents of the tiger-fly, Coenosia attenuata (Diptera: Muscidae), a biological control agent in Mediterranean greenhouses
European Journal of Entomology 118, 335-343
Abstract: The tiger-fly Coenosia attenuata Stein (Diptera: Muscidae: Coenosiini) is a generalist predator that preys on several pests of greenhouse crops and is considered a biological control agent in the Mediterranean region. Previous behavioural observations identified its preferred prey, but a more in-depth evaluation will benefit from using Polymerase Chain Reaction amplification of prey DNA remains in the gut of this predator. To evaluate the rate of decay and suitability of this method for use in the field assessments, we carried out a laboratory feeding calibration experiment on 355 females of C. attenuata, which were killed at different intervals of time after ingestion (10 time points from 0 to 48 h). The prey species tested were: Trialeurodes vaporariorum (Westwood) (Hemiptera: Aleyrodidae: Trialeurodini), Liriomyza huidobrensis (Blanchard) (Diptera: Agromyzidae), Diglyphus isaea (Walker) (Hymenoptera: Eulophidae: Cirrospilini), Bradysia impatiens (Johannsen) (Diptera: Sciaridae) and Drosophila mercatorum Patterson and Wheeler (Diptera: Drosophilidae: Drosophilini). Based on a probit model, amplification success of prey DNA declined exponentially with increasing time after ingestion. The half-time molecular detection differed between species, ranging from an average of 5 h for T. vaporariorum and D. isaea, 6 h for B. impatiens, 15 h for L. huidobrensis to more than 40 h for D. mercatorum. This study confirmed the feasibility of using DNA based detection to identify prey species in the gut contents of C. attenuata and provided calibration curves for a better understanding of predation activity in this agroecosystem.
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Database assignments for author(s): Celia Mateus
Research topic(s) for pests/diseases/weeds:
biocontrol - natural enemies
Research topic(s) for beneficials or antagonists:
general biology - morphology - evolution
