Bulletin of Insectology (2011) 64, S45-S46
Nicoletta Contaldo, Alessandro Canel, Olga Makarova, Samanta Paltrinieri, Assunta Bertaccini and Mogens Nicolaisen (2011)
Use of a fragment of the tuf gene for phytoplasma 16Sr group/subgroup differentiation
Bulletin of Insectology 64 (Suppl.), S45-S46
Second International Phytoplasmologist Working Group Meeting (IPWG), Neustadt an der Weinstraße (Germany) September 12-15, 2011
Abstract: The usefulness of RFLP analyses on a 435 bp fragment of the tuf gene for preliminary identification of phytoplasmas from a number of phytoplasma ribosomal groups and/or `Candidatus Phytoplasma' was verified. The strains employed belong to thirteen 16Sr DNA groups and 22 different subgroups and were obtained from both experimentally and naturally infected plants. The combined RFLP patterns obtained with the three restriction enzymes employed allow the distinction of a total of 18 different profiles, however no discrimination was provided for some of the ribosomal groups for which sequencing remains the main tool for phytoplasma identification.
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Database assignments for author(s): Nicoletta Contaldo, Olga Makarova, Assunta Bertaccini, Mogens Nicolaisen
Research topic(s) for pests/diseases/weeds:
identification/taxonomy
surveys/sampling/distribution
