Applied Entomology and Zoology (2009) 44, 275-280

Kaori Yara and Yasuhisa Kunimi (2009)
Discrimination of Torymus sinensis Kamijo (Hymenoptera: Torymidae) and T. beneficus Yasumatsu et Kamijo and their hybrids by allele-specific PCR
Applied Entomology and Zoology 44 (2), 275-280
Abstract: Torymus sinensis and Torymus beneficus (Hymenoptera: Torymidae) are, respectively, introduced and indigenous parasitoid wasps that attack the invasive chestnut gall wasp, Dryocosmus kuriphilus (Hymenoptera: Cynipidae) in Japan. Torymus beneficus has two emergence types in spring, here designated early-spring and late-spring. It is very difficult to distinguish these Torymus species and emergence types of T. beneficus by their morphological and ecological characteristics. We designed four allele-specific primers for the internal transcribed spacer 1 region of nuclear ribosomal DNA, and developed three kinds of allele-specific PCR. These methods enabled us to distinguish the species, emergence type, and F₁s. This is the first report of the use of molecular markers to distinguish T. sinensis, the late-spring strain of T. beneficus, and their F₁s.
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Database assignments for author(s): Yasuhisa Kunimi

Research topic(s) for pests/diseases/weeds:
biocontrol - natural enemies
Research topic(s) for beneficials or antagonists:
identification/taxonomy

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Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
	Dryocosmus kuriphilus		Japan
	Dryocosmus kuriphilus		China (NE)
Torymus sinensis (parasitoid)	Dryocosmus kuriphilus		Japan
Torymus sinensis (parasitoid)	Dryocosmus kuriphilus		China (NE)
Torymus beneficus (parasitoid)	Dryocosmus kuriphilus		Japan