Annals of Applied Biology (2015) 167, 26-35

I. Ferriol, E.A. Rangel, S. Panno, S. Davino, C.G. Han, A. Olmos and L. Rubio (2015)
Rapid detection and discrimination of fabaviruses by flow-through hybridisation with genus- and species-specific riboprobes
Annals of Applied Biology 167 (1), 26-35
Abstract: Viruses cause significant damage in agricultural crops worldwide. Disease management requires sensitive and specific tools for virus detection and identification. Also, detection techniques need to be rapid to keep pace with the continuous emergence of new viral diseases. The genus Fabavirus is composed of five viruses infecting many economically important crops worldwide. This research describes the development of a procedure based on flow-through hybridisation (FTH), which is faster than and as sensitive as conventional hybridisation for virus detection in tissue-prints from infected plants. Six digoxigenin-labelled RNA probes were synthesised with two levels of specificity: (a) five specific for each viral species within this genus, and (b) a genus-specific probe that hybridises with a nucleotide sequence signature only found in the 5'-untranslated region of the genus Fabavirus, which is the first of this type reported for plant viruses. The new procedure developed is useful for rapid detection and discrimination of the five fabaviruses identified so far and opens the possibility of discovering new species of this genus.
(The abstract is excluded from the Creative Commons licence and has been copied with permission by the publisher.)
Link to article at publishers website
Database assignments for author(s): Luis Rubio, Salvatore Walter Davino

Research topic(s) for pests/diseases/weeds:
surveys/sampling/distribution

---

Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
	Fabavirus alphaviciae
	Fabavirus betaviciae
	Fabavirus gentianae
	Fabavirus cucurbitaceae