Acta Entomologica Sinica (2007) 50, 957-961

Yan-Ling Xu, Zhen-Ying Wang, Kang-Lai He and Shu-Xiong Bai (2007)
[Tissue distribution and content of Cry1Ab insecticidal protein in the Bacillus thuringiensis resistant and susceptible Asian corn borer larvae fed on Bt-transgenic corn]
Acta Entomologica Sinica 50 (9), 957-961
Abstract: The distribution and content of Cry1Ab insecticidal protein in different tissues of the Asian corn borer Ostrinia furnacalis (Guenée), when the 3rd instar larvae of Cry1Ab protein resistant and susceptible colonies fed on whorl leaves of Bt-transgenic corn expressing Cry1Ab protein for 3 days were determined and analyzed by enzyme-linked immunosorbent assay (ELISA). The distributions of Cry1Ab in different tissues in the resistant and susceptible larvae were similar, mainly in the midgut tissue and haemolymph. The Cry1Ab contents in the midgut tissues of the resistant larvae with and without contents inside their midguts were 277.2 ng/g and 52.7 ng/g, respectively, significantly higher than those of the susceptible larvae, which were 104.9 ng/g and 40.1 ng/g, respectively. The Cry1Ab contents in the haemolymph of the resistant and susceptible larvae were 93.7 ng/g and 69.5 ng/g, respectively. The Cry1Ab protein in the silkgland and Malpighian tubules in the resistant and susceptible larvae could be detected, but the concentration was quite low. No Cry1Ab protein was detected in the fat body and reproductive organs, pupae, adults and eggs by ELISA method both in the resistant and susceptible larvae fed on the Bt corn. The results suggested that the Cry1Ab protein from larvae feeding on Bt corn could not be biotransferred to the subsequent pupae, adults and eggs.
(The abstract is excluded from the Creative Commons licence and has been copied with permission by the publisher.)
(original language: Chinese)
Database assignments for author(s): Zhen-Ying Wang, Kanglai He

Research topic(s) for pests/diseases/weeds:
biocontrol - natural enemies
Research topic(s) for beneficials or antagonists:
general biology - morphology - evolution
resistance/tolerance/defence of host

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Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
	Ostrinia furnacalis	Maize/corn (Zea mays)
Bacillus thuringiensis genes in crops (entomopathogen)	Ostrinia furnacalis	Maize/corn (Zea mays)
Bacillus thuringiensis Cry1A-toxin (entomopathogen)	Ostrinia furnacalis	Maize/corn (Zea mays)