Acta Entomologica Sinica (2004) 47, 334-341

Gui-Fen Zhang, Fang-Hao Wan, Jian-Ping Guo and Mao-Lin Hou (2004)
[Expression of Bt toxin in transgenic Bt cotton and its transmission through pests Helicoverpa armigera and Aphis gossypii to natural enemy Propylea japonica in cotton plots]
Acta Entomologica Sinica 47 (3), 334-341
Abstract: The expression of Cry1Ac or Cry1Ab toxins in transgenic Bt cotton lines (NUCOTN 33B and GK-12) was detected; its transmission to the target pest insect, the cotton bollworm Helicoverpa armigera (Hübner), the non-target pest, cotton aphis Aphis gossypii Glover, and the ladybird Propylea japonica (Thunberg) was measured; and its effects on the ladybird were compared with the traditional cotton line, Simian 3 (the isogenic non-transformed line of GK-12) as the control, in cotton plots in Nanpi, Hebei, in 2002. The amounts of expressed Bt toxin in parts of transgenic Bt cotton NUCOTN 33B were higher (79.7 - 1390.0 ng/g fresh weight) than those in GK-12 (16.5 - 264.0 ng/g fresh weight). During the blooming period of NUCOTN 33B plants, the order of Bt toxin content was as follows: stigma, anther > ovary, petal > grouped tender tip; the Bt toxin concentrations were similar as well as equal to those in grouped tender tip, when the newly development tender leaf of the 5-7 leaf stage, the young square of the early squaring period and the young boll of the blooming period of NUCOTN 33B plants were tested. During the blooming period of GK-12 plants, the order of Bt toxin concentration was as follows: anther > stigma > petal > grouped tender tip > ovary; the Bt toxin concentrations were similar and equal to those in grouped tender tip, when the newly development tender leaf of 5-7 leaf stage, the young square of early squaring period and the young boll of blooming period of GK-12 plants were assayed.
(The abstract is excluded from the Creative Commons licence and has been copied with permission by the publisher.)
(original language: Chinese)
Database assignments for author(s): Gui-Fen Zhang, Fang Hao Wan, Mao-Lin Hou

Research topic(s) for pests/diseases/weeds:
biocontrol - natural enemies
Research topic(s) for beneficials or antagonists:
genetical engin./transgenic plants

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Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
	Helicoverpa armigera	Cotton (Gossypium)	China (NE)
Bacillus thuringiensis genes in crops (entomopathogen)	Helicoverpa armigera	Cotton (Gossypium)	China (NE)
Bacillus thuringiensis Cry1A-toxin (entomopathogen)	Helicoverpa armigera	Cotton (Gossypium)	China (NE)