Acta Entomologica Sinica (2000) 43, 233-241

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Chuan-Xi Zhang, Xin-Da Lin and Jun Wu (2000)
Chitinase gene of Helicoverpa armigera nucleopolyhedrovirus and the phylogenetic tree of the baculovirus chitinase genes
Acta Entomologica Sinica 43 (3), 233-241
Abstract: The chitinase gene of Helicoverpa armigera single enveloped nucleocapsid nucleopolyhedrovirus (HaSNPV) was amplified by using PCR method. The nucleotide sequence of the coding region was determined. The total length of the coding region was 1713 bp and encodes a 570 amino acid polypeptide with predicted molecular weight of 63.6 kD. The alignment of HaSNPV chitinase amino acid sequence with those of other 7 reported baculovirus chitinases showed that HaSNPV chitinase was most closely related to HzSNPV chitinase, with 90.7% amino acid identity. It also shared a high degree of homology with chitinases of AcMNPV, BmNPV, HcNPV, LdMNPV, OpMNPV and CfMNPV, with 64.4 %, 64.9 % , 64.2 %, 62.9 %, 66.2 % and 61.5 % amino acid identity, respectively. A phylogenetic tree of 8 baculovirus chitinases was drawn by using the PC\GENE program, and compared with the phylogenetic tree based on the polyhedrin amino acid sequences of the same 8 baculoviruses. The result showed that the evolutionary rate of chitinase genes was in some degree different to that of polyhedrin genes.
(The abstract is excluded from the Creative Commons licence and has been copied with permission by the publisher.)
(original language: Chinese)
Database assignments for author(s): Chuan-Xi Zhang

Research topic(s) for pests/diseases/weeds:
biocontrol - natural enemies
Research topic(s) for beneficials or antagonists:
molecular biology - genes
general biology - morphology - evolution

Pest and/or beneficial records:

Beneficial Pest/Disease/Weed Crop/Product Country Quarant.

Helicoverpa armigera
Helicoverpa armigera nucleopolyhedrovirus (entomopathogen) Helicoverpa armigera