Acta Entomologica Sinica (1997) 40, 7-40

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Yan Wang, Cui Ding and Xiuyu Cal (1997)
[The synergistic action between baculoviruses after mixed infection - A primary analysis of viral proteins and nucleic acids]
Acta Entomologica Sinica 40 (1), 7-40
Abstract: AsNPV was mixed with HasNPV, HaNPV and PsNPV separately and the three mixtures were used to infect the larvae of Helicoverpa assulta, H. armigera and Pseudaletia separata respectively. The nuclear polyhedrosis viruses thus obtained were called (AsNPV+HasNPV) -H. assulta, (AsNPV+HaNPV) -H. armigera and (AsNPV+ PsNPV) -P. separata respectively. The polyhedra of the NPVs were in irregular shape and varied distinctly in sizes. The biochemical charcaters of the viruses were investigated and compared with those of HasNPV, HaNPV and PsNPV. The polyhedrin and viral structural polypeptides of the NPVs were examined by using SDS-PAGE. The polyhedrin of all NPVs had molecular weight of about 25 000 dalton. The numbers of the viral structural polypeptides of (AsNPV+HasNPV) -H. assulta, (AsNPV+HaNPV) -H. armigera and (AsNPV +PsNPV) -P. separata were 10, 14, 5 respectively, and their molecular weights were 13 500-98 000, 13 000~88 000, 18 500~52 000 dalton. The analysis of agarose electrophoretic profiles of DNAs restriction endonuclease fragments showed that they were not identical to those of AsNPV, HasNPV, HaNPV and PsNPV. The DNAs of the viruses had changed and resulted in the changes of viral proteins and viral morphogenesis. Agrotis segetum nuclear polyhedrosis virus (AsNPV) can enhance the infection of HasNPV, HaNPV and PsNPV. The enhancement mechanism of AsNPV is worthwhile to be further investigated.
(The abstract is excluded from the Creative Commons licence and has been copied with permission by the publisher.)
(original language: Chinese)


Research topic(s) for pests/diseases/weeds:
biocontrol - natural enemies
Research topic(s) for beneficials or antagonists:
formulation/storage of bioagents
general biology - morphology - evolution


Pest and/or beneficial records:

Beneficial Pest/Disease/Weed Crop/Product Country Quarant.


Helicoverpa armigera
Helicoverpa assulta
Mythimna separata
Helicoverpa armigera nucleopolyhedrovirus (entomopathogen) Helicoverpa armigera
Agrotis segetum NPV (entomopathogen)
Mythimna separata NPV (entomopathogen) Mythimna separata
Helicoverpa assulta NPV (entomopathogen) Helicoverpa assulta