Journal of Chemical Ecology (2009) 35, 154-162

Harminder Pal Singh, Shalinder Kaur, Sunil Mittal, Daizy Rani Batish and Ravinder Kumar Kohli (2009)
Essential oil of Artemisia scoparia inhibits plant growth by generating reactive oxygen species and causing oxidative damage
Journal of Chemical Ecology 35 (2), 154-162
Abstract: We investigated the chemical composition and phytotoxicity of the essential oil extracted from leaves of Artemisia scoparia Waldst. et Kit. (red stem wormwood, Asteraceae). GC/GC-MS analyses revealed 33 chemical constituents representing 99.83% of the oil. The oil, in general, was rich in monoterpenes that constitute 71.6%, with ß-myrcene (29.27%) as the major constituent followed by (+)-limonene (13.3%), (Z)-ß-ocimene (13.37%), and gamma-terpinene (9.51%). The oil and ß-myrcene were evaluated in a dose-response bioassay under laboratory conditions for phytotoxicity against three weeds-Avena fatua, Cyperus rotundus, and Phalaris minor. A significant reduction in germination, seedling growth, and dry matter accumulation was observed in the test weeds. At the lowest treatment of 0.07 mg/ml Artemisia oil, germination was reduced by 39%, 19%, and 10.6% in C. rotundus, P. minor, and A. fatua, respectively. However, the inhibitory effect of ß-myrcene was less. In general, a dose-dependent effect was observed and the growth declined with increasing concentration. Among the three weeds, the inhibitory effect was greatest on C. rotundus, so it was selected for further studies. We explored the explanation for observed growth inhibition in terms of reactive oxygen species (ROS: lipid peroxidation, membrane integrity, and amounts of conjugated dienes and hydrogen peroxide)-induced oxidative stress. Exposure of C. rotundus to Artemisia oil or ß-myrcene enhanced solute leakage, indicating membrane disintegration. There were increased levels of malondialdehyde and hydrogen peroxide, indicating lipid peroxidation and induction of oxidative stress. We conclude that Artemisia oil inhibits plant root growth through generation of ROS-induced oxidative damage.
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Link to article at publishers website
Database assignments for author(s): Harminder P. Singh, Daizy R. Batish

Research topic(s) for pests/diseases/weeds:
control - general

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Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
	Avena fatua (weed)
	Cyperus rotundus (weed)
	Phalaris minor (weed)