View source for Plant Disease (2007) 91, 525-531

{{Publication
|Publication authors=[[Natalia A. Peres|N.A. Peres]], R. Harakava, G.C. Carroll, [[James E. Adaskaveg|J.E. Adaskaveg]] and L.W. Timmer
|Author Page=Natalia A. Peres, James E. Adaskaveg
|Publication date=2007
|dc:title=Comparison of molecular procedures for detection and identification of ''[[Phyllosticta citricarpa|Guignardia citricarpa]]'' and ''G. mangiferae''
|Publication journal=Plant Disease
|prism:volume=91 (5)
|prism:startingPage=525
|Last page=531
|External link=http://dx.doi.org/10.1094/PDIS-91-5-0525
|Publication abstract=Citrus black spot, caused by ''Guignardia citricarpa, ''is a serious fruit spot disease and is widely distributed in Asia, southern Africa, and South America, but does not occur in North America or the Mediterranean region. A nonpathogenic species, ''G. mangiferae,'' is cosmopolitan with a wide host range and can colonize citrus fruit and leaves saprophytically. Detection and identification of ''Guignardia'' spp. on citrus fruit is necessary for epidemiological, management, and regulatory purposes. In this study, we compared published and unpublished polymerase chain reaction primer sets for their specificity and sensitivity in the detection and differentiation of the two ''Guignardia'' spp. All primers evaluated successfully identified the two species using purified DNA from fungal cultures or mycelia as source materials. However, some primer sets were not highly effective in detecting ''G. citricarpa'' when DNA was extracted directly from single characteristic black spot lesions on fruit. Thus, new primer pairs for both species were designed from the internal transcribed spacer region that were highly sensitive and specific for detection of ''G. citricarpa'' using DNA recovered from single lesions on fruit by a rapid DNA extraction procedure.
|AbstractCC=Yes
|Research topic=identification/taxonomy
|Research2P=molecular biology - genes
|Is book=No
}}
{{Pest record
|Pest=Phyllosticta citricarpa
|Quarantined=No
}}
{{Pest record
|Pest=Phyllosticta capitalensis
|Quarantined=No
}}