View source for PLoS ONE (2014) 9 (7 - e103267)

{{Publication
|Publication authors=[[V.C. Pacheco da Silva|Vitor C. Pacheco da Silva]], Aline Bertin, Aurélie Blin, Jean-François Germain, Daniel Bernardi, Guylène Rignol, [[Marcos Botton]] and Thibaut Malausa
|Author Page=V.C. Pacheco da Silva,, Marcos Botton
|Publication date=2014
|dc:title=Molecular and morphological identification of mealybug species (Hemiptera: Pseudococcidae) in Brazilian vineyards
|Publication journal=PLoS ONE
|prism:volume=9 (7 - e103267)
|Publication abstract=Mealybugs (''Hemiptera: Pseudococcidae'') are pests constraining the international trade of Brazilian table grapes. They damage grapes by transmitting viruses and toxins, causing defoliation, chlorosis, and vigor losses and favoring the development of sooty mold. Difficulties in mealybug identification remain an obstacle to the adequate management of these pests. In this study, our primary aim was to identify the principal mealybug species infesting the major table grape-producing regions in Brazil, by morphological and molecular characterization. Our secondary aim was to develop a rapid identification kit based on species-specific Polymerase Chain Reactions, to facilitate the routine identification of the most common pest species. We surveyed 40 sites infested with mealybugs and identified 17 species: ''Dysmicoccus brevipes'' (Cockerell), ''Dysmicoccus sylvarum'' Williams and Granara de Willink, ''Dysmicoccus texensis'' (Tinsley), ''Ferrisia cristinae'' Kaydan and Gullan, ''Ferrisia meridionalis'' Williams, ''Ferrisia terani'' Williams and Granara de Willink, ''Phenacoccus baccharidis'' Williams, ''Phenacoccus parvus'' Morrison, ''Phenacoccus solenopsis'' Tinsley, ''Planococcus citri'' (Risso), ''Pseudococcus viburni'' (Signoret), ''Pseudococcus cryptus'' Hempel, four taxa closely related each of to ''Pseudococcus viburni, Pseudococcus sociabilis'' Hambleton, ''Pseudococcus maritimus'' (Ehrhorn) and ''Pseudococcus meridionalis'' Prado, and one specimen from the genus ''Pseudococcus'' Westwood. The PCR method developed effectively identified five mealybug species of economic interest on grape in Brazil: ''D. brevipes'', ''Pl. citri'', ''Ps. viburni'', ''Ph. solenopsis'' and ''Planococcus ficus'' (Signoret). Nevertheless, it is not possible to assure that this procedure is reliable for taxa that have not been sampled already and might be very closely related to the target species.
|AbstractCC=Yes
|AuthorsAbstract=No
|Dc:language=English
|External link=http://dx.doi.org/10.1371/journal.pone.0103267
|Research topic=surveys/sampling/distribution
|Is book=No
}}
{{Pest record
|Pest=Planococcus citri
|Crop=Grapevine (Vitis)
|Country=Brazil (south)
|Quarantined=No
}}
{{Pest record
|Pest=Pseudococcus viburni
|Crop=Grapevine (Vitis)
|Country=Brazil (south)
|Quarantined=No
}}
{{Pest record
|Pest=Phenacoccus parvus
|Crop=Grapevine (Vitis)
|Country=Brazil (south)
|Quarantined=No
}}
{{Pest record
|Pest=Dysmicoccus brevipes
|Crop=Grapevine (Vitis)
|Country=Brazil (south)
|Quarantined=No
}}
{{Pest record
|Pest=Dysmicoccus brevipes
|Crop=Grapevine (Vitis)
|Country=Brazil (NE)
|Quarantined=No
}}
{{Pest record
|Pest=Pseudococcus cryptus
|Crop=Grapevine (Vitis)
|Country=Brazil (south)
|Quarantined=No
}}
{{Pest record
|Pest=Phenacoccus solenopsis
|Crop=Grapevine (Vitis)
|Country=Brazil (NE)
|Quarantined=No
}}
{{Pest record
|Pest=Dysmicoccus texensis
|Crop=Grapevine (Vitis)
|Country=Brazil (south)
|Quarantined=No
}}