View source for PLoS Genetics (2022) 18 (11 - e1010333)

{{Publication
|Publication authors=Andre H. Kurlovs, Berdien De Beer, Meiyuan Ji, Marilou Vandenhole, Tim De Meyer, René Feyereisen, Richard M. Clark and [[Thomas Van Leeuwen]]
|Author Page=Thomas Van Leeuwen
|Publication date=2022
|dc:title=''Trans''-driven variation in expression is common among detoxification genes in the extreme generalist herbivore ''[[Tetranychus urticae]]''
|Publication journal=PLoS Genetics
|prism:volume=18 (11 - e1010333)
|External link=https://dx.doi.org/10.1371/journal.pgen.1010333
|Publication abstract=The extreme adaptation potential of the generalist herbivore ''Tetranychus urticae'' (the two-spotted spider mite) to pesticides as well as diverse host plants has been associated with clade-specific gene expansions in known detoxifying enzyme families, and with extensive and rapid transcriptional responses. However, how this broad transcriptional potential is regulated remains largely unknown. Using a parental/F1 design in which four inbred strains were crossed to a common inbred strain, we assessed the genetic basis and inheritance of gene expression variation in ''T''. ''urticae''. Mirroring known phenotypic variation in the progenitor strains of the inbreds, we confirmed that the inbred strains we created were genetically distinct, varied markedly in pesticide resistance, and also captured variation in host plant fitness as is commonly observed in this species. By examining differences in gene expression between parents and allele-specific expression in F1s, we found that variation in RNA abundance was more often explained in ''trans'' as compared to ''cis'', with the former associated with dominance in inheritance. Strikingly, in a gene ontology analysis, detoxification genes of the cytochrome P450 monooxygenase (CYP) family, as well as dioxygenases (DOGs) acquired from horizontal gene transfer from fungi, were specifically enriched at the extremes of ''trans''-driven up- and downregulation. In particular, multiple CYPs and DOGs with broad substrate-specificities for pesticides or plant specialized compounds were exceptionally highly upregulated as a result of ''trans''-regulatory variation, or in some cases synergism of ''cis'' and ''trans'', in the most multi-pesticide resistant strains. Collectively, our findings highlight the potential importance of ''trans''-driven expression variation in genes associated with xenobiotic metabolism and host plant use for rapid adaptation in ''T''. ''urticae'', and also suggests modular control of these genes, a regulatory architecture that might ameliorate negative pleiotropic effects.
|AbstractCC=Yes
|Research topic=molecular biology - genes
|Research2P=pesticide resistance of pest
|Is book=No
}}
{{Pest record
|Pest=Tetranychus urticae
|Crop=Potato (Solanum tuberosum)
|Country=Belgium
|Quarantined=No
}}
{{Pest record
|Pest=Tetranychus urticae
|Crop=Beans (Phaseolus)
|Country=Belgium
|Quarantined=No
}}
{{Pest record
|Pest=Tetranychus urticae
|Crop=Rosa (crop)
|Country=Italy
|Quarantined=No
}}