View source for FEMS Microbiology Letters (2010) 305, 42-48

{{Publication
|Publication authors=Ada Viterbo, Udi Landau, Sofia Kim, [[Leonid S. Chernin|Leonid Chernin]] and Ilan Chet
|Author Page=Leonid S. Chernin
|Publication date=2010
|dc:title=Characterization of ACC deaminase from the biocontrol and plant growth-promoting agent ''[[Trichoderma asperellum (antagonist)|Trichoderma asperellum]]'' T203
|Publication journal=FEMS Microbiology Letters
|prism:volume=305 (1)
|prism:startingPage=42
|Last page=48
|DOI=10.1111/j.1574-6968.2010.01910.x
|Publication abstract=1-Aminocyclopropane-1-carboxylate (ACC) deaminase activity was evaluated in the biocontrol and plant growth-promoting fungus ''Trichoderma asperellum'' T203. Fungal cultures grown with ACC as the sole nitrogen source showed high enzymatic activity. The enzyme encoding gene (''Tas''-''acdS'') was isolated, and an average 3.5-fold induction of the gene by 3 mM ACC was detected by real-time PCR. ''Escherichia coli'' bacteria carrying the intron-free cDNA of ''Tas''-''acdS'' cloned into the vector pAlter-EX1 under the control of the ''tac'' promoter revealed specific ACC deaminase (ACCD) activity and the ability to promote canola (''Brassica napus'') root elongation in pouch assays. RNAi silencing of the ACCD gene in ''T. asperellum'' showed decreased ability of the mutants to promote root elongation of canola seedlings. These data suggest a role for ACCD in the plant root growth-promotion effect by ''T. asperellum''.
|AbstractCC=Yes
|Research topic=biocontrol - natural enemies
|Benf research=molecular biology - genes
|Is book=No
}}
{{Beneficial record
|Beneficial=Trichoderma asperellum (antagonist)
}}