View source for Current Microbiology (2011) 62, 38-43

{{Publication
|Publication authors=Saoussen Ben Khedher, [[Nabil Zouari]], Nadia Messaddeq, Patrick Schultz and [[Samir Jaoua]]
|Author Page=Nabil Zouari, Samir Jaoua
|Publication date=2011
|dc:title=Overproduction of delta-endotoxins by sporeless ''[[Bacillus thuringiensis (entomopathogen)|Bacillus thuringiensis]]'' mutants obtained by nitrous acid mutagenesis
|Publication journal=Current Microbiology
|prism:volume=62 (1)
|prism:startingPage=38
|Last page=43
|DOI=10.1007/s00284-010-9670-x
|Publication abstract=Asporogenic and oligosporogenic ''Bacillus'' ''thuringiensis'' mutants having the ability to overproduce insecticidal crystal protein were generated by using nitrous acid (50 mg/ml), as chemical mutagenic agent. Insecticidal crystal proteins produced by asporogenic mutants remained encapsulated within the cells. Delta-endotoxin production by most of mutants was improved compared to the corresponding wild strains BNS3 and a mutant M26. The overproduction by asporogenic and oligosporogenic mutants was attributed to defect in genes involved in sporulation and to random mutations affecting cell metabolism at different pathways and delta-endotoxin synthesis. Sporeless bioinsecticides could be developed based on stable and environmentally safe ''Bacillus'' ''thuringiensis'' mutants.
|AbstractCC=Yes
|Research topic=biocontrol - natural enemies
|Benf research=general biology - morphology - evolution
|Is book=No
}}
{{Beneficial record
|Beneficial=Bacillus thuringiensis (entomopathogen)
}}