View source for Annals of Microbiology (2009) 59, 545-551

{{Publication
|Publication authors=Priyanka Dhar and Gurvinder Kaur
|Publication date=2009
|dc:title=Effects of carbon and nitrogen sources on the induction and repression of chitinase enzyme from ''[[Metarhizium anisopliae (entomopathogen)|Metarhizium anisopliae]]'' isolates
|Publication journal=Annals of Microbiology
|prism:volume=59 (3)
|prism:startingPage=545
|Last page=551
|DOI=10.1007/BF03175144
|Publication abstract=''Metarhizium anisopliae'', an entomopathogenic hyphomycete, is being used effectively in Integrated Pest Management (IPM) system. Foliar application of these fungi is quite satisfactory as it invades its host by adhering to insect cuticles and formation of penetration structures called appresoria, which produces various extracellular enzymes, including chitinase that causes the insect cuticle breaching. The induction and repression mechanism of chitinase activity is not entirely understood and activity of this enzyme is different in response to different carbon and nitrogen sources. This report illustrates the effect of two carbon sources viz. colloidal chitin and dextrose and a nitrogen source, yeast extract on the chitinase production of fourteen ''M. anisopliae'' isolates. The chitinase activity varied among the isolates and the different media used. A high enzymatic activity was observed in the medium containing an extra nitrogen source (yeast extract) followed by the medium containing colloidal chitin as a sole source of carbon and nitrogen. The exochitinase activity and the chitinase activity gel were also studied for the isolates showing high chitinase enzyme production. An array of chitinase isozymes were observed on chitinase activity gel with a common 14.3 kDa enzyme for all the isolates.
|AbstractCC=Yes
|Research topic=biocontrol - natural enemies
|Benf research=molecular biology - genes
|Is book=No
}}
{{Pest record
|Pest=Nilaparvata lugens
|Country=India
|Quarantined=No
}}
{{Pest record
|Pest=Anticarsia gemmatalis
|Country=Mexico
|Quarantined=No
}}
{{Pest record
|Pest=Nephotettix virescens
|Country=Indonesia
|Quarantined=No
}}
{{Beneficial record
|Beneficial=Metarhizium anisopliae (entomopathogen)
|Pest=Nilaparvata lugens
|Country=India
}}
{{Beneficial record
|Beneficial=Metarhizium anisopliae (entomopathogen)
|Pest=Anticarsia gemmatalis
|Country=Mexico
}}
{{Beneficial record
|Beneficial=Metarhizium anisopliae (entomopathogen)
|Pest=Nephotettix virescens
|Country=Indonesia
}}