Virus Genes (2006) 33, 287-292

Oded Cohen, Ozgur Batuman, Gulshan Stanbekova, Teruo Sano, Munir Mawassi and Moshe Bar-Joseph (2006)
Construction of a multiprobe for the simultaneous detection of viroids infecting citrus trees
Virus Genes 33 (3), 287-292
Abstract: Infections with different viroid species are common among cultivated fruit trees and grapevines, and many old-clone citrus varieties contain up to five citrus viroids (CVds) within a single tree. This paper describes the construction of a CVd-Multiprobe consisting of full-length clones of Hop stunt viroid, Citrus exocortis viroid, Citrus bent leaf viroid and CVd-III. The CVd-Multiprobe was tested against RNA transcripts of the four viroids and RNA extracts from plants singly infected with CEVd or HSVd or multiply infected with different CVds. The viroids were effectively diagnosed with the DIG labeled CVd-Multiprobe when tested by Northern hybridization or dot blot analyses. The CVd-Multiprobe does not provide information on the specific viroid resulting in a positive signal. However, this should not be considered as a problem, since most citrus certification programs will discard budwood source trees infected with any of the known CVds.
(The abstract is excluded from the Creative Commons licence and has been copied with permission by the publisher.)
Link to article at publishers website
Database assignments for author(s): Moshe Bar-Joseph, Teruo Sano, Munir Mawassi

Research topic(s) for pests/diseases/weeds:
identification/taxonomy
surveys/sampling/distribution

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Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
	Citrus exocortis viroid	Citrus (genus)
	Hop stunt viroid	Citrus (genus)
	Citrus dwarfing viroid	Citrus (genus)
	Citrus bent leaf viroid	Citrus (genus)